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Applied and Environmental Microbiology, August 2000, p. 3290-3296, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Changes in Bacterial Community Structure in the Colon of Pigs Fed Different Experimental Diets and after Infection with Brachyspira hyodysenteriae

Thomas D. Leser,1,* Rikke Hvid Lindecrona,1 Tim K. Jensen,1 Bent B. Jensen,2 and Kristian Møller1

Danish Veterinary Laboratory, DK-1790 Copenhagen V,1 and Danish Institute of Agricultural Sciences, Research Centre Foulum, DK-8830 Tjele,2 Denmark

Received 14 February 2000/Accepted 30 May 2000

Bacterial communities in the large intestines of pigs were compared using terminal restriction fragment length polymorphism (T-RFLP) analysis targeting the 16S ribosomal DNA. The pigs were fed different experimental diets based on either modified standard feed or cooked rice supplemented with dietary fibers. After feeding of the animals with the experimental diets for 2 weeks, differences in the bacterial community structure in the spiral colon were detected in the form of different profiles of terminal restriction fragments (T-RFs). Some of the T-RFs were universally distributed, i.e., they were found in all samples, while others varied in distribution and were related to specific diets. The reproducibility of the T-RFLP profiles between individual animals within the diet groups was high. In the control group, the profiles remained unchanged throughout the experiment and were similar between two independent but identical experiments. When the animals were experimentally infected with Brachyspira hyodysenteriae, causing swine dysentery, many of the T-RFs fluctuated, suggesting a destabilization of the microbial community.

* Corresponding author. Mailing address: Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 Copenhagen V, Denmark. Phone: 45 3530 0100. Fax: 45 3530 0120. E-mail: tl{at}svs.dk.

Applied and Environmental Microbiology, August 2000, p. 3290-3296, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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