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Applied and Environmental Microbiology, August 2000, p. 3515-3518, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Lipopolysaccharides of Rhizobium etli Strain G12 Act in Potato Roots as an Inducing Agent of Systemic Resistance to Infection by the Cyst Nematode Globodera pallida

M. Reitz,1 K. Rudolph,2 I. Schröder,2 S. Hoffmann-Hergarten,1 J. Hallmann,1 and R. A. Sikora1,*

Institut für Pflanzenkrankheiten, Phytomedizin in Bodenökosystemen, Universität Bonn, D-53115 Bonn,1 and Institut für Pflanzenpathologie und Pflanzenschutz, Universität Göttingen, D-37077 Göttingen,2 Germany

Received 3 December 1999/Accepted 10 April 2000

Recent studies have shown that living and heat-killed cells of the rhizobacterium Rhizobium etli strain G12 induce in potato roots systemic resistance to infection by the potato cyst nematode Globodera pallida. To better understand the mechanisms of induced resistance, we focused on identifying the inducing agent. Since heat-stable bacterial surface carbohydrates such as exopolysaccharides (EPS) and lipopolysaccharides (LPS) are essential for recognition in the symbiotic interaction between Rhizobium and legumes, their role in the R. etli-potato interaction was studied. EPS and LPS were extracted from bacterial cultures, applied to potato roots, and tested for activity as an inducer of plant resistance to the plant-parasitic nematode. Whereas EPS did not affect G. pallida infection, LPS reduced nematode infection significantly in concentrations as low as 1 and 0.1 mg ml-1. Split-root experiments, guaranteeing a spatial separation of inducing agent and challenging pathogen, showed that soil treatments of one half of the root system with LPS resulted in a highly significant (up to 37%) systemic induced reduction of G. pallida infection of potato roots in the other half. The results clearly showed that LPS of R. etli G12 act as the inducing agent of systemic resistance in potato roots.

* Corresponding author. Mailing address: Institut für Pflanzenkrankheiten, Phytomedizin in Bodenökosystemen, Universität Bonn, Nussalle 9, D-53115 Bonn, Germany. Phone: 49 228 732439. Fax: 49 228 732432. E-mail: rsikora{at}uni-bonn.de.

Applied and Environmental Microbiology, August 2000, p. 3515-3518, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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