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Applied and Environmental Microbiology, August 2000, p. 3621-3623, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

PCR Use of Highly Conserved DNA Regions for Identification of Sinorhizobium meliloti

María Sánchez-Contreras, Javier Lloret, Marta Martín, Marta Villacieros, Ildefonso Bonilla, and Rafael Rivilla*

Departamento de Biología, Universidad Autónoma de Madrid, 28049 Madrid, Spain

Received 4 February 2000/Accepted 16 May 2000

A PCR identification method in which four primers that recognize homologous conserved regions in the Sinorhizobium meliloti genome are used was developed and tested. The regions used for identification were the nodbox 4 locus, which is located in one of the symbiotic megaplasmids, and the mucR gene, which is located in the chromosome. The new method was used to establish a collection of S. meliloti strains from polluted soils.

* Corresponding author. Mailing address: Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Phone: 34 913978177. Fax: 34 913978344. E-mail: rafael.rivilla{at}uam.es.

Applied and Environmental Microbiology, August 2000, p. 3621-3623, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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