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Applied and Environmental Microbiology, September 2000, p. 4152-4156, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Construction of a Multihybrid Display System: Flagellar Filaments Carrying Two Foreign Adhesive Peptides

Jarna Tanskanen, Timo K. Korhonen, and Benita Westerlund-Wikström*

Division of General Microbiology, Department of Biosciences, FIN-00014 University of Helsinki, Finland

Received 24 April 2000/Accepted 5 July 2000

A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in Escherichia coli. The inserted peptides were the fibronectin-binding 115-mer D repeat region of Staphylococcus aureus and the 302-mer collagen-binding region of YadA of Yersinia enterocolitica. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an in trans-complemented Delta fliC E. coli strain showed that individual filaments consisted of both recombinant flagellins.

* Corresponding author. Mailing address: Division of General Microbiology, Department of Biosciences, P.O. Box 56, FIN-00014 University of Helsinki, Finland. Phone: 358-9-19159251. Fax: 358-9-19159262. E-mail: Benita.Westerlund{at}Helsinki.Fi.

Applied and Environmental Microbiology, September 2000, p. 4152-4156, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Majander, K., Korhonen, T. K., Westerlund-Wikstrom, B. (2005). Simultaneous Display of Multiple Foreign Peptides in the FliD Capping and FliC Filament Proteins of the Escherichia coli Flagellum. Appl. Environ. Microbiol. 71: 4263-4268 [Abstract] [Full Text]  


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