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Applied and Environmental Microbiology, September 2000, p. 4161-4167, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Plant Genome Complexity May Be a Factor Limiting In Situ the Transfer of Transgenic Plant Genes to the Phytopathogen Ralstonia solanacearum

Franck Bertolla,1,* Regis Pepin,1 Eugenie Passelegue-Robe,2 Eric Paget,2 Andrew Simkin,3 Xavier Nesme,1 and Pascal Simonet1

Laboratoire d'Ecologie Microbienne, UMR CNRS 5557, Université Lyon I, 69622 Villeurbanne Cedex,1 Aventis Cropscience, Biotechnology Department, 69263 Lyon Cedex 09,2 and Génétique Moléculaire des Plantes, UMR CNRS 5575, Université J. Fourier, 38041 Grenoble Cedex,3 France

Received 13 April 2000/Accepted 30 June 2000

The development of natural competence by bacteria in situ is considered one of the main factors limiting transformation-mediated gene exchanges in the environment. Ralstonia solanacearum is a plant pathogen that is also a naturally transformable bacterium that can develop the competence state during infection of its host. We have attempted to determine whether this bacterium could become the recipient of plant genes. We initially demonstrated that plant DNA was released close to the infecting bacteria. We constructed and tested various combinations of transgenic plants and recipient bacteria to show that the effectiveness of such transfers was directly related to the ratio of the complexity of the plant genome to the number of copies of the transgene.

* Corresponding author. Mailing address: Laboratoire d'Ecologie Microbienne du Sol, UMR CNRS 5557, Bâtiment 741, Université Lyon I, 43 bd. du 11 Novembre 1918, F-69622 Villeurbanne Cedex, France. Phone: 33 4 72 44 82 89. Fax: 33 4 72 43 12 23. E-mail: bertolla{at}biomserv.univ-lyon1.fr.

Applied and Environmental Microbiology, September 2000, p. 4161-4167, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ceccherini, M., Pote, J., Kay, E., Van, V. T., Marechal, J., Pietramellara, G., Nannipieri, P., Vogel, T. M., Simonet, P. (2003). Degradation and Transformability of DNA from Transgenic Leaves. Appl. Environ. Microbiol. 69: 673-678 [Abstract] [Full Text]  
  • Kay, E., Vogel, T. M., Bertolla, F., Nalin, R., Simonet, P. (2002). In Situ Transfer of Antibiotic Resistance Genes from Transgenic (Transplastomic) Tobacco Plants to Bacteria. Appl. Environ. Microbiol. 68: 3345-3351 [Abstract] [Full Text]  


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