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Applied and Environmental Microbiology, January 2001, p. 449-452, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.449-452.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Magnetic Bead Capture Eliminates PCR Inhibitors in Samples Collected from the Airborne Environment, Permitting Detection of Pneumocystis carinii DNA

Nancy Maher, H. Kenneth Dillon, Sten H. Vermund, and Thomas R. Unnasch*

Division of Geographic Medicine and Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, Alabama

Received 4 May 2000/Accepted 29 September 2000

PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.

* Corresponding author. Mailing address: Division of Geographic Medicine, University of Alabama at Birmingham, BBRB 203, 845 19th St. South, Birmingham, AL 35294-2170. Phone: (205) 975-7601. Fax: (205) 933-5671. E-mail: trunnasch{at}geomed.dom.uab.edu.

Applied and Environmental Microbiology, January 2001, p. 449-452, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.449-452.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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