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Applied and Environmental Microbiology, January 2001, p. 459-461, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.459-461.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Application of Rapid Dot Blot Immunoassay for Detection of Salmonella enterica Serovar Enteritidis in Eggs, Poultry, and Other Foods

Mark Akira Yoshimasudagger and Jerzy Zawistowski*

Department of Food Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2

Received 31 May 2000/Accepted 20 October 2000

Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Serovar Enteritidis was detected in the presence of other bacteria when outcompeted 1:400.

* Corresponding author. Present address: Forbes Medi-Tech Inc., Suite 200, 750 West Pender St., Vancouver, BC, V6C 2T8, Canada. Phone: (604) 689-5899. Fax: (604) 689-7641. E-mail: jzawistowski{at}forbesmedi.com.

dagger Present address: Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1.

Applied and Environmental Microbiology, January 2001, p. 459-461, Vol. 67, No. 1
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.1.459-461.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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