This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Küsel, K.
Right arrow Articles by Drake, H. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Küsel, K.
Right arrow Articles by Drake, H. L.
Agricola
Right arrow Articles by Küsel, K.
Right arrow Articles by Drake, H. L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2001, p. 4734-4741, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4734-4741.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Physiological Ecology of Clostridium glycolicum RD-1, an Aerotolerant Acetogen Isolated from Sea Grass Roots

Kirsten Küsel,1,* Arno Karnholz,1 Tanja Trinkwalter,1 Richard Devereux,2 Georg Acker,3 and Harold L. Drake1

Department of Ecological Microbiology, BITOEK,1 and Electron Microscopy Laboratory,3 University of Bayreuth, 95440 Bayreuth, Germany, and Gulf Ecology Division, US EPA/NHEERL, Gulf Breeze, Florida 325612

Received 24 April 2001/Accepted 31 July 2001

An anaerobic, H2-utilizing bacterium, strain RD-1, was isolated from the highest growth-positive dilution series of a root homogenate prepared from the sea grass Halodule wrightii. Cells of RD-1 were gram-positive, spore-forming, motile rods that were linked by connecting filaments. Acetate was produced in stoichiometries indicative of an acetyl coenzyme A (acetyl-CoA) pathway-dependent metabolism when RD-1 utilized H2-CO2, formate, lactate, or pyruvate. Growth on sugars or ethylene glycol yielded acetate and ethanol as end products. RD-1 grew at the expense of glucose in the presence of low initial concentrations (up to 6% [vol/vol]) of O2 in the headspace of static, horizontally incubated culture tubes; the concentration of O2 decreased during growth in such cultures. Peroxidase, NADH oxidase, and superoxide dismutase activities were detected in the cytoplasmic fraction of cells grown in the presence of O2. In comparison to cultures incubated under strictly anoxic conditions, acetate production decreased, higher amounts of ethanol were produced, and lactate and H2 became significant end products when RD-1 was grown on glucose in the presence of O2. Similarly, when RD-1 was grown on fructose in the presence of elevated salt concentrations, lower amounts of acetate and higher amounts of ethanol and H2 were produced. When the concentration of O2 in the headspace exceeded 1% (vol/vol), supplemental H2 was not utilized. The 16S rRNA gene of RD-1 had a 99.7% sequence similarity to that of Clostridium glycolicum DSM 1288T, an organism characterized as a fermentative anaerobe. Comparative experiments with C. glycolicum DSM 1288T demonstrated that it had negligible H2- and formate-utilizing capacities. However, carbon monoxide dehydrogenase was detected in both RD-1 and C. glycolicum DSM 1288T. A 91.4% DNA-DNA hybridization between the genomic DNA of RD-1 and that of C. glycolicum DSM 1288T confirmed that RD-1 was a strain of C. glycolicum. These results indicate that (i) RD-1 metabolizes certain substrates via the acetyl-CoA pathway, (ii) RD-1 can tolerate and consume limited amounts of O2, (iii) oxic conditions favor the production of ethanol, lactate, and H2 by RD-1, and (iv) the ability of RD-1 to cope with limited amounts of O2 might contribute to its survival in a habitat subject to daily gradients of photosynthesis-derived O2.

* Corresponding author. Mailing address: Department of Ecological Microbiology, BITOEK, University of Bayreuth, 95440 Bayreuth, Germany. Phone: [49] (0)921-555 642. Fax: [49] (0)921-555 799. E-mail: kirsten.kuesel{at}bitoek.uni-bayreuth.de.

Applied and Environmental Microbiology, October 2001, p. 4734-4741, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4734-4741.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jiang, W., Abrar, S., Romagnoli, M., Carroll, K. C. (2009). Clostridium glycolicum Wound Infections: Case Reports and Review of the Literature. J. Clin. Microbiol. 47: 1599-1601 [Abstract] [Full Text]  
  • Balk, M., van Gelder, T., Weelink, S. A., Stams, A. J. M. (2008). (Per)chlorate Reduction by the Thermophilic Bacterium Moorella perchloratireducens sp. nov., Isolated from Underground Gas Storage. Appl. Environ. Microbiol. 74: 403-409 [Abstract] [Full Text]  
  • Elsayed, S., Zhang, K. (2007). Clostridium glycolicum Bacteremia in a Bone Marrow Transplant Patient. J. Clin. Microbiol. 45: 1652-1654 [Abstract] [Full Text]  
  • Kawasaki, S., Mimura, T., Satoh, T., Takeda, K., Niimura, Y. (2006). Response of the Microaerophilic Bifidobacterium Species, B. boum and B. thermophilum, to Oxygen. Appl. Environ. Microbiol. 72: 6854-6858 [Abstract] [Full Text]  
  • Gossner, A. S., Kusel, K., Schulz, D., Trenz, S., Acker, G., Lovell, C. R., Drake, H. L. (2006). Trophic interaction of the aerotolerant anaerobe Clostridium intestinale and the acetogen Sporomusa rhizae sp. nov. isolated from roots of the black needlerush Juncus roemerianus.. Microbiology 152: 1209-1219 [Abstract] [Full Text]  
  • Kawasaki, S., Watamura, Y., Ono, M., Watanabe, T., Takeda, K., Niimura, Y. (2005). Adaptive Responses to Oxygen Stress in Obligatory Anaerobes Clostridium acetobutylicum and Clostridium aminovalericum. Appl. Environ. Microbiol. 71: 8442-8450 [Abstract] [Full Text]  
  • Horn, M. A., Ihssen, J., Matthies, C., Schramm, A., Acker, G., Drake, H. L. (2005). Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa. Int. J. Syst. Evol. Microbiol. 55: 1255-1265 [Abstract] [Full Text]  
  • Das, A., Silaghi-Dumitrescu, R., Ljungdahl, L. G., Kurtz, D. M. Jr. (2005). Cytochrome bd Oxidase, Oxidative Stress, and Dioxygen Tolerance of the Strictly Anaerobic Bacterium Moorella thermoacetica. J. Bacteriol. 187: 2020-2029 [Abstract] [Full Text]  
  • Abboud, R., Popa, R., Souza-Egipsy, V., Giometti, C. S., Tollaksen, S., Mosher, J. J., Findlay, R. H., Nealson, K. H. (2005). Low-Temperature Growth of Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 71: 811-816 [Abstract] [Full Text]  
  • Kato, S., Haruta, S., Cui, Z. J., Ishii, M., Yokota, A., Igarashi, Y. (2004). Clostridium straminisolvens sp. nov., a moderately thermophilic, aerotolerant and cellulolytic bacterium isolated from a cellulose-degrading bacterial community. Int. J. Syst. Evol. Microbiol. 54: 2043-2047 [Abstract] [Full Text]  
  • Boga, H. I., Ludwig, W., Brune, A. (2003). Sporomusa aerivorans sp. nov., an oxygen-reducing homoacetogenic bacterium from the gut of a soil-feeding termite. Int. J. Syst. Evol. Microbiol. 53: 1397-1404 [Abstract] [Full Text]  
  • Boga, H. I., Brune, A. (2003). Hydrogen-Dependent Oxygen Reduction by Homoacetogenic Bacteria Isolated from Termite Guts. Appl. Environ. Microbiol. 69: 779-786 [Abstract] [Full Text]  
  • Karnholz, A., Kusel, K., Go{beta}ner, A., Schramm, A., Drake, H. L. (2002). Tolerance and Metabolic Response of Acetogenic Bacteria toward Oxygen. Appl. Environ. Microbiol. 68: 1005-1009 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»