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Applied and Environmental Microbiology, October 2001, p. 4817-4827, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4817-4827.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cloning and Genetic Characterization of dca Genes Required for beta -Oxidation of Straight-Chain Dicarboxylic Acids in Acinetobacter sp. Strain ADP1†

D. Parke,1,* M. A. Garcia,2 and L. N. Ornston1

Department of Molecular, Cellular, and Developmental Biology1 and Department of Ecology and Evolutionary Biology,2 Yale University, New Haven, Connecticut 06520-8103

Received 23 February 2001/Accepted 7 June 2001

A previous study of deletions in the protocatechuate (pca) region of the Acinetobacter sp. strain ADP1 chromosome revealed that genes required for utilization of the six-carbon dicarboxylic acid, adipic acid, are linked to the pca structural genes. To investigate the genes involved in adipate catabolism, a 33.8-kb SacI fragment, which corrects a deletion spanning this region, was cloned. In addition to containing known pca, qui, and pob genes (for protocatechuate, quinate, and 4-hydroxybenzoate dissimilation), clone pZR8000 contained 10 kb of DNA which was the subject of this investigation. A mutant strain of Escherichia coli DH5alpha , strain EDP1, was isolated that was able to utilize protocatechuate and 4-hydroxybenzoate as growth substrates when EDP1 cells contained pZR8000. Sequence analysis of the new region of DNA on pZR8000 revealed open reading frames predicted to be involved in beta -oxidation. Knockouts of three genes implicated in beta -oxidation steps were introduced into the chromosome of Acinetobacter sp. strain ADP1. Each of the mutants was unable to grow with adipate. Because the mutants were affected in their ability to utilize additional saturated, straight-chain dicarboxylic acids, the newly discovered 10 kb of DNA was termed the dca (dicarboxylic acid) region. Mutant strains included one with a deletion in dcaA (encoding an acyl coenzyme A [acyl-CoA] dehydrogenase homolog), one with a deletion in dcaE (encoding an enoyl-CoA hydratase homolog), and one with a deletion in dcaH (encoding a hydroxyacyl-CoA dehydrogenase homolog). Data on the dca region should help us probe the functional significance and interrelationships of clustered genetic elements in this section of the Acinetobacter chromosome.

* Corresponding author. Mailing address: Department of Molecular, Cellular, and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520-8103. Phone: (203) 432-3505. Fax: (203) 432-6161. E-mail: donna.parke{at}yale.edu.

dagger This article is publication number 28 from the Biological Transformation Center in the Yale Institute for Biospheric Studies.

Applied and Environmental Microbiology, October 2001, p. 4817-4827, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4817-4827.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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