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Applied and Environmental Microbiology, October 2001, p. 4914-4918, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4914-4918.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Availability of Glutamate and Arginine during Acid Challenge Determines Cell Density-Dependent Survival Phenotype of Escherichia coli Strains

Shenghui Cui,^1,2 Jianghong Meng,² and Arvind A. Bhagwat^1,*

Produce Quality and Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, Maryland 20705-2350,¹ and Department of Nutrition and Food Science, University of Maryland, College Park, Maryland 20742²

Received 18 April 2001/Accepted 29 June 2001

The cell density-dependent acid sensitivity phenotypes of Escherichia coli strains K-12 and O157:H7 were examined with reference to three possible mechanisms of acid resistance. There was no evidence of any diffusible substance released from dead cells which could influence the cell density-dependent acid survival phenotype. Instead, cell density-dependent acid survival phenotype was associated with induction of glutamate- and arginine-decarboxylase acid survival pathways and concomitant availability of glutamate and arginine during acid challenge.

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^* Corresponding author. Mailing address: Produce Quality and Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg. 002, 10300 Baltimore Avenue, Beltsville, MD 20705-2350. Phone: (301) 504-5106. Fax: (301) 504-5107. E-mail: bhagwata{at}ba.ars.usda.gov.

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Applied and Environmental Microbiology, October 2001, p. 4914-4918, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4914-4918.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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