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Applied and Environmental Microbiology, October 2001, p. 4939-4942, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4939-4942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparative Study of Bacterial Groups within the Human Cecal and Fecal Microbiota

Philippe Marteau,1,2,* Philippe Pochart,1,3 Joël Doré,4 Christel Béra-Maillet,4 Annick Bernalier,5 and Gérard Corthier4

INSERM U 290, Paris,1 Département de gastroentérologie, Hôpital Européen Georges Pompidou, AP-HP, 75908 Paris,2 Laboratoire de Biologie, Conservatoire National des Arts et Métiers, 75003 Paris,3 INRA, CR de Jouy-en-Josas, 78352 Jouy en Josas,4 and Laboratoire de Microbiologie, INRA, CR de Clermont-Ferrand/Theix, 63122 Saint Genes-Champanelle,5 France

Received 2 April 2001/Accepted 1 August 2001

The composition of the human cecal microbiota is poorly known because of sampling difficulties. Samples of cecal fluid from eight subjects were collected via an intestinal tube. Feces were also collected. Total anaerobes, facultative anaerobes, bifidobacteria, and Bacteroides were enumerated by culture methods, and the predominant phylogenetic groups were quantified by molecular hybridization using a set of six rRNA-targeted probes. The numbers of strict anaerobes, bifidobacteria, Bacteroides, and members of the Clostridium coccoides group and Clostridium leptum subgroup were lower in the cecum. Facultative anaerobes represented 25% of total bacteria in the cecum versus 1% in the feces.

* Corresponding author. Mailing address: Département de gastroentérologie, Hôpital Européen Georges Pompidou, 20 rue Leblanc, 75908, Paris CEDEX 15, France. Phone: 33 1 5609 3555. Fax: 33 1 5609 3554. E-mail: philippe.marteau{at}egp.ap-hop-paris.fr.

Applied and Environmental Microbiology, October 2001, p. 4939-4942, Vol. 67, No. 10
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.10.4939-4942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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