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Applied and Environmental Microbiology, November 2001, p. 5328-5330, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5328-5330.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Bacillus thuringiensis Delta-Endotoxin Cry1 Hybrid Proteins with Increased Activity against the Colorado Potato Beetle

Samir Naimov,1 Mieke Weemen-Hendriks,2 Stefan Dukiandjiev,1 and Ruud A. de Maagd2,*

Business Unit Cell Cybernetics, Plant Research International B.V., 6700 AA Wageningen, The Netherlands,2 and Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria1

Received 7 June 2001/Accepted 13 August 2001

Cry1 delta-endotoxins of Bacillus thuringiensis are generally active against lepidopteran insects, but Cry1Ba and Cry1Ia have additional, though low, levels of activity against coleopterans such as the Colorado potato beetle. Here we report the construction of Cry1Ba/Cry1Ia hybrid toxins which have increased activities against this insect species.

* Corresponding author. Mailing address: Business Unit Cell Cybernetics, Plant Research International B.V., P.O. Box 16, 6700 AA Wageningen, The Netherlands. Phone: 31(317) 477128. Fax: 31(317) 418094. E-mail: R.A.deMaagd{at}plant.wag-ur.nl.

Applied and Environmental Microbiology, November 2001, p. 5328-5330, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5328-5330.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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