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Applied and Environmental Microbiology, November 2001, p. 5331-5334, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5331-5334.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8

M. Julia Pettinari,1 Gustavo J. Vázquez,1 Daniel Silberschmidt,1,dagger Bernd Rehm,2 Alexander Steinbüchel,2 and Beatriz S. Méndez1,*

Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina,1 and Institut für Mikrobiologie, Westfälische Wilhems-Universität Münster, D-48149 Münster, Germany2

Received 13 June 2001/Accepted 1 September 2001

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta -ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.

* Corresponding author. Mailing address: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina. Phone: 54-11-4576-3334. Fax: 54-11-4576-3342. E-mail: bea{at}qb.fcen.uba.ar.

dagger Present address: Laboratory of Biochemistry and Molecular Biology, Stazione Zoologica A. Dohrn, 80121 Naples, Italy.

Applied and Environmental Microbiology, November 2001, p. 5331-5334, Vol. 67, No. 11
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.11.5331-5334.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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