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Applied and Environmental Microbiology, December 2001, p. 5506-5511, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5506-5511.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Homologous Expression of the Lipase and ABC Transporter Gene Cluster, tliDEFA, Enhances Lipase Secretion in Pseudomonas spp.

Jung Hoon Ahn,1 Jae Gu Pan,2 and Joon Shick Rhee3,*

R&D Center, Creagene Inc., Seo-gu, Taejon 302-8581; Genofocus Inc., Yusong-gu, Taejon 305-3902; and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yusong-gu, Taejon 305-701,3 Korea

Received 14 May 2001/Accepted 25 September 2001

The ABC transporter TliDEF was found to be an efficient secretory apparatus for extracellular lipase TliA in Pseudomonas fluorescens. For the enhanced secretion of the lipase, we tried to coexpress tliA and tliDEF in various Pseudomonas species. Whereas the coexpression of tliA and tliDEF was required for the lipase secretion in P. fragi, the expression of tliA was sufficient for the lipase secretion in P. fluorescens, P. syringae, and P. putida, indicating the existence of compatible ABC transporter in these species. However, P. fluorescens harboring tliDEFA secreted much more lipase than P. fluorescens harboring only tliA, but the tliDEF was functional only at temperatures below 30°C. The recombinant P. fluorescens overexpressing tliDEFA showed the highest secretion level, 217 U/ml · OD (optical density) (28 µg/ml · OD) of lipase in Luria-Bertani medium under microaerated conditions. With the increase of aeration, the lipase production was decreased and the lipase seemed to be degraded as the cells entered the cell death phase. These results demonstrate that P. fluorescens can be used as a host system for the secretory production of the lipase using the ABC transporter, thus producing lipase in over 14% of the total protein.

* Corresponding author. Mailing address: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yusong-gu, Taejon 305-701, Korea. Phone: 82-42-869-2613. Fax: 82-42-869-2610. E-mail: jsrhee{at}mail.kaist.ac.kr.

Applied and Environmental Microbiology, December 2001, p. 5506-5511, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5506-5511.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Eom, G. T., Song, J. K., Ahn, J. H., Seo, Y. S., Rhee, J. S. (2005). Enhancement of the Efficiency of Secretion of Heterologous Lipase in Escherichia coli by Directed Evolution of the ABC Transporter System. Appl. Environ. Microbiol. 71: 3468-3474 [Abstract] [Full Text]  


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