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Applied and Environmental Microbiology, December 2001, p. 5601-5607, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5601-5607.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Biodegradation of Methyl tert-Butyl Ether by a Pure Bacterial Culture

Paul B. Hatzinger, Kevin McClay, Simon Vainberg, Marina Tugusheva, Charles W. Condee, and Robert J. Steffan*

Envirogen, Inc., Lawrenceville, New Jersey 08648

Received 23 April 2001/Accepted 1 October 2001

Biodegradation of methyl tert-butyl ether (MTBE) by the hydrogen-oxidizing bacterium Hydrogenophaga flava ENV735 was evaluated. ENV735 grew slowly on MTBE or tert-butyl alcohol (TBA) as sole sources of carbon and energy, but growth on these substrates was greatly enhanced by the addition of a small amount of yeast extract. The addition of H2 did not enhance or diminish MTBE degradation by the strain, and MTBE was only poorly degraded or not degraded by type strains of Hydrogenophaga or hydrogen-oxidizing enrichment cultures, respectively. MTBE degradation activity was constitutively expressed in ENV735 and was not greatly affected by formaldehyde, carbon monoxide, allyl thiourea, or acetylene. MTBE degradation was inhibited by 1-amino benzotriazole and butadiene monoepoxide. TBA degradation was inducible by TBA and was inhibited by formaldehyde at concentrations of >0.24 mM and by acetylene but not by the other inhibitors tested. These results demonstrate that separate, independently regulated genes encode MTBE and TBA metabolism in ENV735.

* Corresponding author. Mailing address: Envirogen, Inc., 4100 Quakerbridge Rd., Lawrenceville, N.J. 08648. Phone: (609) 936-9300. Fax: (609) 936-9221. E-mail: Steffan{at}envirogen.com.

Applied and Environmental Microbiology, December 2001, p. 5601-5607, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5601-5607.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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