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Applied and Environmental Microbiology, December 2001, p. 5833-5839, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5833-5839.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

An Antifungal Exo-alpha -1,3-Glucanase (AGN13.1) from the Biocontrol Fungus Trichoderma harzianum

Hassane Ait-Lahsen,1 Andrés Soler,1 Manuel Rey,1 Jesús de la Cruz,1,dagger Enrique Monte,2 and Antonio Llobell1,*

Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Seville,1 and Centro Hispano-Luso de Investigaciones Agrarias, Universidad de Salamanca, Salamanca,2 Spain

Received 15 June 2001/Accepted 30 September 2001

Trichoderma harzianum secretes alpha -1,3-glucanases when it is grown on polysaccharides, fungal cell walls, or autoclaved mycelium as a carbon source (simulated antagonistic conditions). We have purified and characterized one of these enzymes, named AGN13.1. The enzyme was monomeric and slightly basic. AGN13.1 was an exo-type alpha -1,3-glucanase and showed lytic and antifungal activity against fungal plant pathogens. Northern and Western analyses indicated that AGN13.1 is induced by conditions that simulated antagonism. We propose that AGN13.1 contributes to the antagonistic response of T. harzianum.

* Corresponding author. Mailing address: Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Avda. Américo Vespucio s/n, Isla de la Cartuja, E-41092 Seville, Spain. Phone: 34 95 4489521. Fax: 34 95 4460065. E-mail: Llobell{at}cica.es.

dagger Present address: Departamento de Genética, Facultad de Biología, Universidad de Sevilla. Seville, Spain.

Applied and Environmental Microbiology, December 2001, p. 5833-5839, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5833-5839.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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