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Applied and Environmental Microbiology, December 2001, p. 5840-5843, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5840-5843.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Typing by Pulsed-Field Gel Electrophoresis of Spanish Animal and Human Listeria monocytogenes Isolates

A. I. Vela,1 J. F. Fernandez-Garayzabal,1,* J. A. Vazquez,2 M. V. Latre,3 M. M. Blanco,1 M. A. Moreno,1 L. de la Fuente,2 J. Marco,4 C. Franco,5 A. Cepeda,5 A. A. Rodriguez Moure,3 G. Suarez,1 and L. Dominguez1

Departamento de Sanidad Animal (Patología Animal I), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid,1 Servicio de Bacteriología, Centro Nacional de Microbiología-Instituto de Salud Carlos III, Majadahonda, 28220 Madrid,2 Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza,3 Laboratorio Normativo de Salud Pública. C. M. Díaz de Haro, 60, 48010 Bilbao,4 and Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo,5 Spain

Received 26 April 2001/Accepted 4 September 2001

A total of 153 strains of Listeria monocytogenes isolated from different sources (72 from sheep, 12 from cattle, 18 from feedstuffs, and 51 from humans) in Spain from 1989 to 2000 were characterized by pulsed-field gel electrophoresis. The strains of L. monocytogenes displayed 55 pulsotypes. The 84 animal, 51 human, and 18 feedstuff strains displayed 31, 29, and 7 different pulsotypes, respectively, indicating a great genetic diversity among the Spanish L. monocytogenes isolates studied. L. monocytogenes isolates from clinical samples and feedstuffs consumed by the diseased animals were analyzed in 21 flocks. In most cases, clinical strains from different animals of the same flock had identical pulsotypes, confirming the existence of a listeriosis outbreak. L. monocytogenes strains with pulsotypes identical to those of clinical strains were isolated from silage, potatoes, and maize stalks. This is the first study wherein potatoes and maize stalks are epidemiologically linked with clinical listeriosis.

* Corresponding author. Mailing address: Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. Phone: 34-91-3943716. Fax: 34-91-3943908. E-mail: garayzab{at}eucmax.sim.ucm.es.

Applied and Environmental Microbiology, December 2001, p. 5840-5843, Vol. 67, No. 12
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.12.5840-5843.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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