This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Whitford, M. F.
Right arrow Articles by Teather, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Whitford, M. F.
Right arrow Articles by Teather, R. M.
Agricola
Right arrow Articles by Whitford, M. F.
Right arrow Articles by Teather, R. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2001, p. 569-574, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.569-574.2001

Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255†

M. F. Whitford,1 M. A. McPherson,2 R. J. Forster,1 and R. M. Teather1,*

Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada T1J 4B1,1 and Biological Sciences Department, University of Alberta, Edmonton, Alberta, Canada T6G 2E92

Received 13 September 2000/Accepted 5 November 2000

Streptococci obtained from rumen sources were tested for the production of antibacterial compounds using a deferred-antagonism plating assay. Of 35 isolates tested, 7 were identified that inhibited the growth of other streptococci. None of the inhibitory activity was due to bacteriophage. Three isolates, LRC0253, LRC0255, and LRC0476, were selected for further characterization. Analysis of 16S ribosomal DNA indicated that LRC0476 was a strain of Streptococcus bovis, while isolates LRC0253 and LRC0255 are likely strains of Streptococcus gallolyticus. The antibacterial compounds produced by these bacteria were protease sensitive, remained active in a pH range from 1 to 12, and did not lose activity after heating at 100°C for 15 min. The inhibitory peptide from strain LRC0255 was purified using pH-dependent adsorption and desorption to bacterial cells, followed by ammonium sulfate precipitation and reversed-phase chromatography and gel filtration. The peptide was 6 kDa, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. An oligonucleotide probe based on the N-terminal sequence of the purified peptide was used to identify the gene encoding the inhibitory peptide. The antibacterial peptide has characteristics that are very similar to those described for class II bacteriocins of gram-positive bacteria.

* Corresponding author. Mailing address: Lethbridge Research Centre, Agriculture and Agri-Food Canada, P.O. Box 3000 Lethbridge, Alberta, Canada T1J 4B1. Phone: (403) 317-2246. Fax: (403) 382-3156. E-mail: teather{at}em.agr.ca.

dagger Contribution number 3870074 of the Lethbridge Research Centre.

Applied and Environmental Microbiology, February 2001, p. 569-574, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.569-574.2001


This article has been cited by other articles:

  • Nes, I. F., Diep, D. B., Holo, H. (2007). Bacteriocin Diversity in Streptococcus and Enterococcus. J. Bacteriol. 189: 1189-1198 [Full Text]  
  • He, Z., Kisla, D., Zhang, L., Yuan, C., Green-Church, K. B., Yousef, A. E. (2007). Isolation and Identification of a Paenibacillus polymyxa Strain That Coproduces a Novel Lantibiotic and Polymyxin. Appl. Environ. Microbiol. 73: 168-178 [Abstract] [Full Text]  
  • Chen, J., Stevenson, D. M., Weimer, P. J. (2004). Albusin B, a Bacteriocin from the Ruminal Bacterium Ruminococcus albus 7 That Inhibits Growth of Ruminococcus flavefaciens. Appl. Environ. Microbiol. 70: 3167-3170 [Abstract] [Full Text]  
  • Xiao, H., Chen, X., Chen, M., Tang, S., Zhao, X., Huan, L. (2004). Bovicin HJ50, a novel lantibiotic produced by Streptococcus bovis HJ50. Microbiology 150: 103-108 [Abstract] [Full Text]  
  • Mantovani, H. C., Hu, H., Worobo, R. W., Russell, J. B. (2002). Bovicin HC5, a bacteriocin from Streptococcus bovis HC5. Microbiology 148: 3347-3352 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»