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Applied and Environmental Microbiology, February 2001, p. 769-773, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.769-773.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mobilization of Selenite by Ralstonia metallidurans CH34

Murielle Roux,1 Géraldine Sarret,2 Isabelle Pignot-Paintrand,3 Marc Fontecave,1 and Jacques Coves1,*

Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, CEA-Grenoble, DBMS/CB-CNRS-Université Joseph Fourier,1 and Atelier de Microscopie Electronique, IFR 27 INSERM, CEA-Grenoble,3 38054 Grenoble Cedex 9, and Laboratoire de Géophysique Interne et Tectonophysique, Université Joseph Fourier, 38041 Grenoble Cedex,2 France

Received 3 August 2000/Accepted 22 November 2000

Ralstonia metallidurans CH34 (formerly Alcaligenes eutrophus CH34) is a soil bacterium characteristic of metal-contaminated biotopes, as it is able to grow in the presence of a variety of heavy metals. R. metallidurans CH34 is reported now to resist up to 6 mM selenite and to reduce selenite to elemental red selenium as shown by extended X-ray absorption fine-structure analysis. Growth kinetics analysis suggests an adaptation of the cells to the selenite stress during the lag-phase period. Depending on the culture conditions, the medium can be completely depleted of selenite. Selenium accumulates essentially in the cytoplasm as judged from electron microscopy and energy-dispersive X-ray analysis. Elemental selenium, highly insoluble, represents a nontoxic storage form for the bacterium. The ability of R. metallidurans CH34 to reduce large amounts of selenite may be of interest for bioremediation processes targeting selenite-polluted sites.

* Corresponding author. Mailing address: Laboratoire de Chimie et Biochimie des Centres Redox Biologiques, CEA-Grenoble, DBMS/CB-CNRS-Université Joseph Fourier, 38054 Grenoble Cedex 9, France. Phone: 33-(0)4-76-88-91-22. Fax: 33-(0)4-76-88-91-24. E-mail: jcoves{at}cea.fr.

Applied and Environmental Microbiology, February 2001, p. 769-773, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.769-773.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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