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Applied and Environmental Microbiology, February 2001, p. 969-971, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.969-971.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Production of Capsular Polysaccharide of Streptococcus pneumoniae Type 14 and Its Purification by Affinity Chromatography

Norma Suárez,1 Laura Franco Fraguas,2 Esther Texeira,3 Hugo Massaldi,3 Francisco Batista-Viera,2 and Fernando Ferreira1,*

Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Quimica Orgánica, Facultad de Química,1 and Departamento de Desarrollo y Producción, Facultad de Medicina,3 Instituto de Higiene, C.P. 11600, and Facultad de Química, Cátedra de Bioquímica, CC 1157,2 Montevideo, Uruguay

Received 23 December 1999/Accepted 20 October 2000

We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular polysaccharides from other streptococcal serotypes by selecting the appropriate lectin adsorbents.

* Corresponding author. Mailing address: Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Desarrollo y Producción, Instituto de Higiene, C.P. 11600, Av. A. Navarro 3051, Montevideo, Uruguay. Phone: (598) 2 4871288. Fax: (598) 2 4873073. E-mail: fferreir{at}bilbo.edu.uy.

Applied and Environmental Microbiology, February 2001, p. 969-971, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.969-971.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chaffin, D. O., Mentele, L. M., Rubens, C. E. (2005). Sialylation of Group B Streptococcal Capsular Polysaccharide Is Mediated by cpsK and Is Required for Optimal Capsule Polymerization and Expression. J. Bacteriol. 187: 4615-4626 [Abstract] [Full Text]  


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