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Applied and Environmental Microbiology, February 2001, p. 977-978, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.977-978.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Twelve-Hour PCR-Based Method for Detection of Salmonella spp. in Food

R. Ferretti,1,* I. Mannazzu,1 L. Cocolin,2 G. Comi,2 and F. Clementi1

Dipartimento di Biotecnologie Agrarie ed Ambientali, 60100 Ancona,1 and Dipartimento di Scienze degli Alimenti, 33100 Udine,2 Italy

Received 24 July 2000/Accepted 13 November 2000

A PCR-based method for the detection of Salmonella spp. in food was developed. The method, set up on typical salami from the Italian region of Marche, is sensitive and specific and shows excellent correlation with the conventional method of reference when naturally contaminated foods are analyzed. Moreover, it can be easily performed within a maximum of 12 h from food sampling, thus allowing prompt detection of Salmonella spp. in the food stocks analyzed.

* Corresponding author. Mailing address: Dipartimento di Biotecnologie Agrarie ed Ambientali, Via Brecce Bianche, 60100 Ancona, Italy. Phone: +390 71 2204782. Fax: +390 71 2204858. E-mail: anmicro{at}popcsi.unian.it.

Applied and Environmental Microbiology, February 2001, p. 977-978, Vol. 67, No. 2
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.2.977-978.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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