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Applied and Environmental Microbiology, March 2001, p. 1371-1374, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1371-1374.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Oligophilic Bacteria as Tools To Monitor Aseptic Pharmaceutical Production Units

Parag P. Nagarkar,¹ Satish D. Ravetkar,¹ and Milind G. Watve^2,*

Research Laboratory, Research Foundation, Serum Institute of India, Hadapsar,¹ and Department of Microbiology, Abasaheb Garware College,² Pune, India

Received 5 September 2000/Accepted 12 December 2000

The bacterial loads of air, surfaces, and personnel in clean rooms are routinely monitored using a set of standard media. Bacteria that can grow on these media are a tiny fraction of the total numbers in any environment. A substantial proportion of bacteria long thought to be unculturable were recently shown to be oligophilic. Oligophile counts in clean rooms in our studies exceeded the standard plate counts by up to 2 orders of magnitude. They responded to disinfection routines in ways similar to the responses of conventional bacteria. We suggest that oligophiles are better tools than conventional bacteria for environmental monitoring in aseptic pharmaceutical production units.

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^* Corresponding author. Mailing address: Department of Microbiology, Abasaheb Garware College, Karve Rd., Pune 411 004, India. Phone: 91-20-5440311. Fax: 91-20-4338009. E-mail: watve{at}vsnl.com.

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Applied and Environmental Microbiology, March 2001, p. 1371-1374, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1371-1374.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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