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Applied and Environmental Microbiology, March 2001, p. 1396-1399, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1396-1399.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Reductive, Coenzyme A-Mediated Pathway for 3-Chlorobenzoate Degradation in the Phototrophic Bacterium Rhodopseudomonas palustris

Paul G. Egland,dagger Jane Gibson, and Caroline S. Harwood*

Department of Microbiology, University of Iowa, Iowa City, Iowa 52242

Received 23 October 2000/Accepted 1 January 2001

We isolated a strain of Rhodopseudomonas palustris (RCB100) by selective enrichment in light on 3-chlorobenzoate to investigate the steps that it uses to accomplish anaerobic dechlorination. Analyses of metabolite pools as well as enzyme assays suggest that R. palustris grows on 3-chlorobenzoate by (i) converting it to 3-chlorobenzoyl coenzyme A (3-chlorobenzoyl-CoA), (ii) reductively dehalogenating 3-chlorobenzoyl-CoA to benzoyl-CoA, and (iii) degrading benzoyl-CoA to acetyl-CoA and carbon dioxide. R. palustris uses 3-chlorobenzoate only as a carbon source and thus incorporates the acetyl-CoA that is produced into cell material. The reductive dechlorination route used by R. palustris for 3-chlorobenzoate degradation differs from those previously described in that a CoA thioester, rather than an unmodified aromatic acid, is the substrate for complete dehalogenation.

* Corresponding author. Mailing address: Department of Microbiology, 3-432 BSB, The University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7783. Fax: (319) 335-7679. E-mail: caroline-harwood{at}uiowa.edu.

dagger Present address: National Institutes of Health, NIDCR, Bethesda, MD 20892-4350.

Applied and Environmental Microbiology, March 2001, p. 1396-1399, Vol. 67, No. 3
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.3.1396-1399.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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