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Applied and Environmental Microbiology, April 2001, p. 1675-1681, Vol. 67, No. 4
Microbial Biotechnology, Tata Energy Research
Institute,1 and Department of
Microbiology, University of Delhi South
Campus,2 New Delhi, India
Received 14 November 2000/Accepted 1 February 2001
A full-scale study evaluating an inoculum addition to stimulate in
situ bioremediation of oily-sludge-contaminated soil was conducted at
an oil refinery where the indigenous population of hydrocarbon-degrading bacteria in the soil was very low
(103 to 104 CFU/g of soil). A
feasibility study was conducted prior to the full-scale bioremediation
study. In this feasibility study, out of six treatments, the
application of a bacterial consortium and nutrients resulted in maximum
biodegradation of total petroleum hydrocarbon (TPH) in 120 days.
Therefore, this treatment was selected for the full-scale study. In the
full-scale study, plots A and B were treated with a bacterial
consortium and nutrients, which resulted in 92.0 and 89.7% removal of
TPH, respectively, in 1 year, compared to 14.0% removal of TPH in the
control plot C. In plot A, the alkane fraction of TPH was reduced
by 94.2%, the aromatic fraction of TPH was reduced by 91.9%,
and NSO (nitrogen-, sulfur-, and oxygen-containing compound) and
asphaltene fractions of TPH were reduced by 85.2% in 1 year.
Similarly, in plot B the degradation of alkane, aromatic, and NSO plus
asphaltene fractions of TPH was 95.1, 94.8, and 63.5%, respectively,
in 345 days. However, in plot C, removal of alkane (17.3%), aromatic
(12.9%), and NSO plus asphaltene (5.8%) fractions was much less. The
population of introduced Acinetobacter baumannii strains
in plots A and B was stable even after 1 year. Physical and chemical
properties of the soil at the bioremediation site improved
significantly in 1 year.
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.4.1675-1681.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Evaluation of Inoculum Addition To Stimulate In
Situ Bioremediation of Oily-Sludge-Contaminated Soil
*
Corresponding author. Mailing address: Microbial
Biotechnology, Tata Energy Research Institute, Habitat Place, Lodhi
Rd., New Delhi 110 003, India. Phone: 91 11 4682100 or 4682111. Fax: 91 11 4682144 or 4682145. E-mail: banwaril{at}teri.res.in.
Applied and Environmental Microbiology, April 2001, p. 1675-1681, Vol. 67, No. 4
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.4.1675-1681.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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