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Applied and Environmental Microbiology, April 2001, p. 1693-1699, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1693-1699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sensitivities of Germinating Spores and Carvacrol-Adapted Vegetative Cells and Spores of Bacillus cereus to Nisin and Pulsed-Electric-Field Treatment

Irene E. Pol,* Willy G. C. van Arendonk, Hennie C. Mastwijk, Judit Krommer,dagger Eddy J. Smid,Dagger and Roy Moezelaar

Agrotechnological Research Institute (ATO), 6708 PD Wageningen, The Netherlands

Received 20 October 2000/Accepted 29 January 2001

Treatment of Bacillus cereus spores with nisin and/or pulsed-electric-field (PEF) treatment did not lead to direct inactivation of the spores or increased heat sensitivity as a result of sublethal damage. In contrast, germinating spores were found to be sensitive to PEF treatment. Nisin treatment was more efficient than PEF treatment for inactivating germinating spores. PEF resistance was lost after 50 min of germination, and not all germinated spores could be inactivated. Nisin, however, was able to inactivate the germinating spores to the same extent as heat treatment. Resistance to nisin was lost immediately when the germination process started. A decrease in the membrane fluidity of vegetative cells caused by incubation in the presence of carvacrol resulted in a dramatic increase in the sensitivity to nisin. On the other hand, inactivation by PEF treatment or by a combination of nisin and PEF treatments did not change after adaptation to carvacrol. Spores grown in the presence of carvacrol were not susceptible to nisin and/or PEF treatment in any way.

* Corresponding author. Mailing address: Department Preservation Technology and Food Safety, ATO, Bornsesteeg 59, 6708 GA Wageningen, The Netherlands. Phone: 31.317.475108. Fax: 31.317.475.347. E-mail: I.E.Pol{at}ATO.WAG-UR.NL.

dagger Present address: Hungarian Meat Research Institute, 1097 Budapest, Hungary.

Dagger Present address: NIZO Food Research, 6710 BA Ede, The Netherlands.

Applied and Environmental Microbiology, April 2001, p. 1693-1699, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1693-1699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gut, I. M., Prouty, A. M., Ballard, J. D., van der Donk, W. A., Blanke, S. R. (2008). Inhibition of Bacillus anthracis Spore Outgrowth by Nisin. Antimicrob. Agents Chemother. 52: 4281-4288 [Abstract] [Full Text]  


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