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Applied and Environmental Microbiology, April 2001, p. 1959-1963, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1959-1963.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Methyl Halide-Utilizing Genes in the Methyl Bromide-Utilizing Bacterial Strain IMB-1 Suggests a High Degree of Conservation of Methyl Halide-Specific Genes in Gram-Negative Bacteria

Claire A. Woodall,1 Karen L. Warner,1 Ronald S. Oremland,2 J. Colin Murrell,1 and Ian R. McDonald1,*

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England,1 and U.S. Geological Survey, Menlo Park, California 940252

Received 14 November 2000/Accepted 22 January 2001

Strain IMB-1, an aerobic methylotrophic member of the alpha subgroup of the Proteobacteria, can grow with methyl bromide as a sole carbon and energy source. A single cmu gene cluster was identified in IMB-1 that contained six open reading frames: cmuC, cmuA, orf146, paaE, hutI, and partial metF. CmuA from IMB-1 has high sequence homology to the methyltransferase CmuA from Methylobacterium chloromethanicum and Hyphomicrobium chloromethanicum and contains a C-terminal corrinoid-binding motif and an N-terminal methyltransferase motif. However, cmuB, identified in M. chloromethanicum and H. chloromethanicum, was not detected in IMB-1.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England. Phone: 44 24 765 28362. Fax: 44 24 765 23568. E-mail: imcdonald{at}bio.warwick.ac.uk.

Applied and Environmental Microbiology, April 2001, p. 1959-1963, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1959-1963.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Borodina, E., McDonald, I. R., Murrell, J. C. (2004). Chloromethane-Dependent Expression of the cmu Gene Cluster of Hyphomicrobium chloromethanicum. Appl. Environ. Microbiol. 70: 4177-4186 [Abstract] [Full Text]  
  • Studer, A., McAnulla, C., Buchele, R., Leisinger, T., Vuilleumier, S. (2002). Chloromethane-Induced Genes Define a Third C1 Utilization Pathway in Methylobacterium chloromethanicum CM4. J. Bacteriol. 184: 3476-3484 [Abstract] [Full Text]  
  • Goodwin, K. D., Varner, R. K., Crill, P. M., Oremland, R. S. (2001). Consumption of Tropospheric Levels of Methyl Bromide by C1 Compound-Utilizing Bacteria and Comparison to Saturation Kinetics. Appl. Environ. Microbiol. 67: 5437-5443 [Abstract] [Full Text]  
  • Miller, L. G., Kalin, R. M., McCauley, S. E., Hamilton, J. T. G., Harper, D. B., Millet, D. B., Oremland, R. S., Goldstein, A. H. (2001). Large carbon isotope fractionation associated with oxidation of methyl halides by methylotrophic bacteria. Proc. Natl. Acad. Sci. USA 98: 5833-5837 [Abstract] [Full Text]  


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