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Applied and Environmental Microbiology, April 2001, p. 1964-1969, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1964-1969.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cloning and Expression of a Ralstonia eutropha HF39 Gene Mediating Indigo Formation in Escherichia coli

Sascha Drewlo,1 Christian O. Brämer,1 Mohamed Madkour,2 Frank Mayer,2 and Alexander Steinbüchel1,*

Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, D-48149 Münster,1 and Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, D-37073, Göttingen,2 Germany

Received 10 November 2000/Accepted 17 January 2001

On complex medium Escherichia coli strains carrying hybrid plasmid pBEC/EE:11.0, pSKBEC/BE:9.0, pSKBEC/PP:3.3, or pSKBEC/PP:2.4 harboring genomic DNA of Ralstonia eutropha HF39 produced a blue pigment characterized as indigo by several chemical and spectroscopic methods. A 1,251-bp open reading frame (bec) was cloned and sequenced. The deduced amino acid sequence of bec showed only weak similarities to short-chain acyl-coenzyme A dehydrogenases, and the gene product catalyzed formation of indoxyl, a reactive preliminary stage for production of indigo.

* Corresponding author. Mailing address: Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Münster, Germany. Phone: 49-251-8339821. Fax: 49-251-8338388. E-mail: steinbu{at}uni-muenster.de.

Applied and Environmental Microbiology, April 2001, p. 1964-1969, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1964-1969.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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