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Applied and Environmental Microbiology, April 2001, p. 1979-1982, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1979-1982.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Inhibition of a Glucose-Limited Sequencing Fed-Batch Culture of Salmonella enterica Serovar Enteritidis by Volatile Fatty Acids Representative of the Ceca of Broiler Chickens

Paul W. J. J. van der Wielen,1,* Steef Biesterveld,2 Len J. A. Lipman,1 and Frans van Knapen1

Centre for Veterinary Public Health and Environmental Protection, Faculty of Veterinary Medicine, Utrecht University, Utrecht,1 and Campina Melkunie, Zaltbommel,2 The Netherlands

Received 2 October 2000/Accepted 5 February 2001

The effects of concentrations of volatile fatty acids on an anaerobic, glucose-limited, and pH-controlled growing culture of Salmonella enterica serovar Enteritidis were studied. Suddenly increasing volatile fatty acids to the concentrations representative of the ceca of 15-day-old broiler chickens caused washout of serovar Enteritidis. In contrast, a sudden increase to the volatile fatty acid concentrations representative of the ceca of younger broiler chickens caused a reduction in the biomass but not washout. Gradually increasing volatile fatty acids caused a gradual decrease in the biomass of serovar Enteritidis. We conclude that the concentrations of volatile fatty acids present in the ceca of broilers with a mature microflora can cause washout of serovar Enteritidis in an in vitro system mimicking cecal ecophysiology.

* Corresponding author. Mailing address: CVVM, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80175, 3508 TD Utrecht, The Netherlands. Phone: 31 30 253 33 79. Fax: 31 30 253 23 65. E-mail: wielen{at}vvdo.vet.uu.nl.

Applied and Environmental Microbiology, April 2001, p. 1979-1982, Vol. 67, No. 4
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.4.1979-1982.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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