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Applied and Environmental Microbiology, May 2001, p. 2145-2155, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2145-2155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Predator-Specific Enrichment of Actinobacteria from a Cosmopolitan Freshwater Clade in Mixed Continuous Culture

Jakob Pernthaler,1,* Thomas Posch,2 Karel Simek,3,4 Jaroslav Vrba,3 Annelie Pernthaler,1 Frank Oliver Glöckner,1 Ulrich Nübel,1,dagger Roland Psenner,2 and Rudolf Amann1

Max Planck Institute for Marine Microbiology, D-28359 Bremen, Germany1; Institute of Zoology and Limnology, A-6020 Innsbruck, Austria2; and Hydrobiological Institute of the Czech Academy of Sciences3 and Faculty of Biological Sciences, University of South Bohemia,4 CS-37005 Ceské Budejovice, Czech Republic

Received 26 October 2000/Accepted 15 February 2001

We investigated whether individual populations of freshwater bacteria in mixed experimental communities may exhibit specific responses to the presence of different bacterivorous protists. In two successive experiments, a two-stage continuous cultivation system was inoculated with nonaxenic batch cultures of the cryptophyte Cryptomonas sp. Algal exudates provided the sole source of organic carbon for growth of the accompanying microflora. The dynamics of several 16S rRNA-defined bacterial populations were followed in the experimental communities. Although the composition and stability of the two microbial communities differed, numerous members of the first assemblage could again be detected during the second experiment. The introduction of a size-selectively feeding mixotrophic nanoflagellate (Ochromonas sp.) always resulted in an immediate bloom of a single phylotype population of members of the class Actinobacteria (Ac1). These bacteria were phylogenetically affiliated with an uncultured lineage of gram-positive bacteria that have been found in freshwater habitats only. The Ac1 cells were close to the average size of freshwater bacterioplankton and significantly smaller than any of the other experimental community members. In contrast, no increase of the Ac1 population was observed in vessels exposed to the bacterivorous ciliate Cyclidium glaucoma. However, when the Ochromonas sp. was added after the establishment of C. glaucoma, the proportion of population Ac1 within the microbial community rapidly increased. Populations of a beta proteobacterial phylotype related to an Aquabacterium sp. decreased relative to the total bacterial communities following the addition of either predator, albeit to different extents. The community structure of pelagic microbial assemblages can therefore be influenced by the taxonomic composition of the predator community.

* Corresponding author. Mailing address: Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany. Phone: 0494212028940. Fax: 0494212028580. E-mail: jperntha{at}mpi-bremen.de.

dagger Present address: DSMZ, D-38124 Braunschweig, Germany.

Applied and Environmental Microbiology, May 2001, p. 2145-2155, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2145-2155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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