This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Uhlich, G. A.
Right arrow Articles by Elder, R. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uhlich, G. A.
Right arrow Articles by Elder, R. O.
Agricola
Right arrow Articles by Uhlich, G. A.
Right arrow Articles by Elder, R. O.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2001, p. 2367-2370, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2367-2370.2001

Mutations in the csgD Promoter Associated with Variations in Curli Expression in Certain Strains of Escherichia coli O157:H7

Gaylen A. Uhlich,* James E. Keen, and Robert O. Elderdagger

Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933

Received 9 October 2000/Accepted 16 February 2001

Single-base-pair csgD promoter mutations in human outbreak Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 coincided with differential Congo red dye binding from curli fiber expression. Red phenotype csgD::lacZ promoter fusions had fourfold-greater expression than white promoter fusions. Cloning the red variant csgDEFG operon into white variants induced the red phenotype. Substrate utilization differed between red and white variants.

* Corresponding author. Present address: USDA, ARS, Eastern Regional Research Center, 600 East Mermaid Ln., Wyndmoor, PA 19038. Phone: (215) 233-6740. Fax: (215) 233-6581. E-mail: guhlich{at}arserrc.gov.

dagger Present address: USDA, ARS, Southern Plains Agricultural Research Center, College Station, TX 77845.

Applied and Environmental Microbiology, May 2001, p. 2367-2370, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2367-2370.2001


This article has been cited by other articles:

  • Uhlich, G. A. (2009). KatP contributes to OxyR-regulated hydrogen peroxide resistance in Escherichia coli serotype O157 : H7. Microbiology 155: 3589-3598 [Abstract] [Full Text]  
  • Uhlich, G. A., Gunther, N. W. IV, Bayles, D. O., Mosier, D. A. (2009). The CsgA and Lpp Proteins of an Escherichia coli O157:H7 Strain Affect HEp-2 Cell Invasion, Motility, and Biofilm Formation. Infect. Immun. 77: 1543-1552 [Abstract] [Full Text]  
  • Rosser, T., Dransfield, T., Allison, L., Hanson, M., Holden, N., Evans, J., Naylor, S., La Ragione, R., Low, J. C., Gally, D. L. (2008). Pathogenic Potential of Emergent Sorbitol-Fermenting Escherichia coli O157:NM. Infect. Immun. 76: 5598-5607 [Abstract] [Full Text]  
  • Gualdi, L., Tagliabue, L., Bertagnoli, S., Ierano, T., De Castro, C., Landini, P. (2008). Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli. Microbiology 154: 2017-2024 [Abstract] [Full Text]  
  • Uhlich, G. A., Sinclair, J. R., Warren, N. G., Chmielecki, W. A., Fratamico, P. (2008). Characterization of Shiga Toxin-Producing Escherichia coli Isolates Associated with Two Multistate Food-Borne Outbreaks That Occurred in 2006. Appl. Environ. Microbiol. 74: 1268-1272 [Abstract] [Full Text]  
  • Dyer, J. G., Sriranganathan, N., Nickerson, S. C., Elvinger, F. (2007). Curli Production and Genetic Relationships Among Escherichia coli from Cases of Bovine Mastitis. J DAIRY SCI 90: 193-201 [Abstract] [Full Text]  
  • White, A. P., Surette, M. G. (2006). Comparative Genetics of the rdar Morphotype in Salmonella. J. Bacteriol. 188: 8395-8406 [Abstract] [Full Text]  
  • Uhlich, G. A., Cooke, P. H., Solomon, E. B. (2006). Analyses of the Red-Dry-Rough Phenotype of an Escherichia coli O157:H7 Strain and Its Role in Biofilm Formation and Resistance to Antibacterial Agents. Appl. Environ. Microbiol. 72: 2564-2572 [Abstract] [Full Text]  
  • Low, A. S., Dziva, F., Torres, A. G., Martinez, J. L., Rosser, T., Naylor, S., Spears, K., Holden, N., Mahajan, A., Findlay, J., Sales, J., Smith, D. G. E., Low, J. C., Stevens, M. P., Gally, D. L. (2006). Cloning, Expression, and Characterization of Fimbrial Operon F9 from Enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 74: 2233-2244 [Abstract] [Full Text]  
  • Brombacher, E., Baratto, A., Dorel, C., Landini, P. (2006). Gene Expression Regulation by the Curli Activator CsgD Protein: Modulation of Cellulose Biosynthesis and Control of Negative Determinants for Microbial Adhesion. J. Bacteriol. 188: 2027-2037 [Abstract] [Full Text]  
  • Torres, A. G., Jeter, C., Langley, W., Matthysse, A. G. (2005). Differential Binding of Escherichia coli O157:H7 to Alfalfa, Human Epithelial Cells, and Plastic Is Mediated by a Variety of Surface Structures. Appl. Environ. Microbiol. 71: 8008-8015 [Abstract] [Full Text]  
  • Bokranz, W., Wang, X., Tschape, H., Romling, U. (2005). Expression of cellulose and curli fimbriae by Escherichia coli isolated from the gastrointestinal tract. J Med Microbiol 54: 1171-1182 [Abstract] [Full Text]  
  • Barak, J. D., Gorski, L., Naraghi-Arani, P., Charkowski, A. O. (2005). Salmonella enterica Virulence Genes Are Required for Bacterial Attachment to Plant Tissue. Appl. Environ. Microbiol. 71: 5685-5691 [Abstract] [Full Text]  
  • Yap, M.-N., Yang, C.-H., Barak, J. D., Jahn, C. E., Charkowski, A. O. (2005). The Erwinia chrysanthemi Type III Secretion System Is Required for Multicellular Behavior. J. Bacteriol. 187: 639-648 [Abstract] [Full Text]  
  • Ryu, J.-H., Beuchat, L. R. (2005). Biofilm Formation by Escherichia coli O157:H7 on Stainless Steel: Effect of Exopolysaccharide and Curli Production on Its Resistance to Chlorine. Appl. Environ. Microbiol. 71: 247-254 [Abstract] [Full Text]  
  • Brombacher, E., Dorel, C., Zehnder, A. J. B., Landini, P. (2003). The curli biosynthesis regulator CsgD co-ordinates the expression of both positive and negative determinants for biofilm formation in Escherichia coli. Microbiology 149: 2847-2857 [Abstract] [Full Text]  
  • White, A. P., Gibson, D. L., Collinson, S. K., Banser, P. A., Kay, W. W. (2003). Extracellular Polysaccharides Associated with Thin Aggregative Fimbriae of Salmonella enterica Serovar Enteritidis. J. Bacteriol. 185: 5398-5407 [Abstract] [Full Text]  
  • Torres, A. G., Kaper, J. B. (2003). Multiple Elements Controlling Adherence of Enterohemorrhagic Escherichia coli O157:H7 to HeLa Cells. Infect. Immun. 71: 4985-4995 [Abstract] [Full Text]  
  • Zogaj, X., Bokranz, W., Nimtz, M., Romling, U. (2003). Production of Cellulose and Curli Fimbriae by Members of the Family Enterobacteriaceae Isolated from the Human Gastrointestinal Tract. Infect. Immun. 71: 4151-4158 [Abstract] [Full Text]  
  • Chirwa, N. T., Herrington, M. B. (2003). CsgD, a regulator of curli and cellulose synthesis, also regulates serine hydroxymethyltransferase synthesis in Escherichia coli K-12. Microbiology 149: 525-535 [Abstract] [Full Text]  
  • COOKSON, A. L., WALES, A. D., ROE, J. M., HAYES, C. M., PEARSON, G. R., WOODWARD, M. J. (2002). Variation in the persistence of Escherichia coli O157:H7 in experimentally inoculated 6-week-old conventional lambs. J Med Microbiol 51: 1032-1040 [Abstract] [Full Text]  
  • Barak, J. D., Whitehand, L. C., Charkowski, A. O. (2002). Differences in Attachment of Salmonella enterica Serovars and Escherichia coli O157:H7 to Alfalfa Sprouts. Appl. Environ. Microbiol. 68: 4758-4763 [Abstract] [Full Text]  
  • Uhlich, G. A., Keen, J. E., Elder, R. O. (2002). Variations in the csgD Promoter of Escherichia coli O157:H7 Associated with Increased Virulence in Mice and Increased Invasion of HEp-2 Cells. Infect. Immun. 70: 395-399 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»