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Applied and Environmental Microbiology, May 2001, p. 2375-2379, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2375-2379.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

16S Ribosomal DNA Characterization of Nitrogen-Fixing Bacteria Isolated from Banana (Musa spp.) and Pineapple (Ananas comosus (L.) Merril)

Leonardo Magalhães Cruz,1 Emanuel Maltempi de Souza,1 Olmar Baler Weber,2 José Ivo Baldani,3 Johanna Döbereiner,3 and Fábio de Oliveira Pedrosa1,*

Departamento de Bioquímica, Universidade Federal do Paraná, 81.531-990 Curitiba, Paraná,1 Centro Nacional de Agroindústria Tropical-Empresa Brasileira de Pesquisa Agropecuária, 60.511-110 Fortaleza, Ceará,2 and Centro Nacional de Pesquisa de Agrobiologia-Empresa Brasileira de Pesquisa Agropecuária, 23.851-970 Seropédica, Rio de Janeiro,3 Brazil

Received 4 April 2000/Accepted 7 February 2001

Nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril) were characterized by amplified 16S ribosomal DNA restriction analysis and 16S rRNA sequence analysis. Herbaspirillum seropedicae, Herbaspirillum rubrisubalbicans, Burkholderia brasilensis, and Burkholderia tropicalis were identified. Eight other types were placed in close proximity to these genera and other alpha and beta Proteobacteria.

* Corresponding author. Mailing address: Departamento de Bioquímica, Universidade Federal do Paraná (UFPR), C. Postal 19046, 81.531-990, Curitiba, Paraná, Brazil. Phone: 55 41 3664398. Fax: 55 41 2662042. E-mail: fpedrosa{at}bio.ufpr.br.

Applied and Environmental Microbiology, May 2001, p. 2375-2379, Vol. 67, No. 5
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.5.2375-2379.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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