This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Estrada-De Los Santos, P.
Right arrow Articles by Caballero-Mellado, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Estrada-De Los Santos, P.
Right arrow Articles by Caballero-Mellado, J.
Agricola
Right arrow Articles by Estrada-De Los Santos, P.
Right arrow Articles by Caballero-Mellado, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2001, p. 2790-2798, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2790-2798.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Burkholderia, a Genus Rich in Plant-Associated Nitrogen Fixers with Wide Environmental and Geographic Distribution

Paulina Estrada-De Los Santos, Rocío Bustillos-Cristales, and Jesús Caballero-Mellado*

P. de Ecología Molecular y Microbiana, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico

Received 27 November 2000/Accepted 12 March 2001

The genus Burkholderia comprises 19 species, including Burkholderia vietnamiensis which is the only known N2-fixing species of this bacterial genus. The first isolates of B. vietnamiensis were recovered from the rhizosphere of rice plants grown in a phytotron, but its existence in natural environments and its geographic distribution were not reported. In the present study, most N2-fixing isolates recovered from the environment of field-grown maize and coffee plants cultivated in widely separated regions of Mexico were phenotypically identified as B. cepacia using the API 20NE system. Nevertheless, a number of these isolates recovered from inside of maize roots, as well as from the rhizosphere and rhizoplane of maize and coffee plants, showed similar or identical features to those of B. vietnamiensis TVV75T. These features include nitrogenase activity with 10 different carbon sources, identical or very similar nifHDK hybridization patterns, very similar protein electrophoregrams, identical amplified 16S rDNA restriction (ARDRA) profiles, and levels of DNA-DNA reassociation higher than 70% with total DNA from strain TVV75T. Although the ability to fix N2 is not reported to be a common feature among the known species of the genus Burkholderia, the results obtained show that many diazotrophic Burkholderia isolates analyzed showed phenotypic and genotypic features different from those of the known N2-fixing species B. vietnamiensis as well as from those of B. kururiensis, a bacterium identified in the present study as a diazotrophic species. DNA-DNA reassociation assays confirmed the existence of N2-fixing Burkholderia species different from B. vietnamiensis. In addition, this study shows the wide geographic distribution and substantial capability of N2-fixing Burkholderia spp. for colonizing diverse host plants in distantly separated environments.

* Corresponding author. Mailing address: Centro de Investigación sobre Fijación de Nitrógeno, UNAM, Ap. Postal No. 565-A, Cuernavaca, Morelos, Mexico. Fax: (73) 17-55-81. E-mail: jesuscab{at}cifn.unam.mx.

Applied and Environmental Microbiology, June 2001, p. 2790-2798, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2790-2798.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Onofre-Lemus, J., Hernandez-Lucas, I., Girard, L., Caballero-Mellado, J. (2009). ACC (1-Aminocyclopropane-1-Carboxylate) Deaminase Activity, a Widespread Trait in Burkholderia Species, and Its Growth-Promoting Effect on Tomato Plants. Appl. Environ. Microbiol. 75: 6581-6590 [Abstract] [Full Text]  
  • Escalante, A. E., Caballero-Mellado, J., Martinez-Aguilar, L., Rodriguez-Verdugo, A., Gonzalez-Gonzalez, A., Toribio-Jimenez, J., Souza, V. (2009). Pseudomonas cuatrocienegasensis sp. nov., isolated from an evaporating lagoon in the Cuatro Cienegas valley in Coahuila, Mexico. Int. J. Syst. Evol. Microbiol. 59: 1416-1420 [Abstract] [Full Text]  
  • Martinez-Aguilar, L., Diaz, R., Pena-Cabriales, J. J., Estrada-de los Santos, P., Dunn, M. F., Caballero-Mellado, J. (2008). Multichromosomal Genome Structure and Confirmation of Diazotrophy in Novel Plant-Associated Burkholderia Species. Appl. Environ. Microbiol. 74: 4574-4579 [Abstract] [Full Text]  
  • Suarez-Moreno, Z. R., Caballero-Mellado, J., Venturi, V. (2008). The new group of non-pathogenic plant-associated nitrogen-fixing Burkholderia spp. shares a conserved quorum-sensing system, which is tightly regulated by the RsaL repressor. Microbiology 154: 2048-2059 [Abstract] [Full Text]  
  • Elliott, G. N., Chen, W.-M., Bontemps, C., Chou, J.-H., Young, J. P. W., Sprent, J. I., James, E. K. (2007). Nodulation of Cyclopia spp. (Leguminosae, Papilionoideae) by Burkholderia tuberum. ANN BOT (LOND) 100: 1403-1411 [Abstract] [Full Text]  
  • Mendes, R., Pizzirani-Kleiner, A. A., Araujo, W. L., Raaijmakers, J. M. (2007). Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates. Appl. Environ. Microbiol. 73: 7259-7267 [Abstract] [Full Text]  
  • Caballero-Mellado, J., Onofre-Lemus, J., Estrada-de los Santos, P., Martinez-Aguilar, L. (2007). The Tomato Rhizosphere, an Environment Rich in Nitrogen-Fixing Burkholderia Species with Capabilities of Interest for Agriculture and Bioremediation. Appl. Environ. Microbiol. 73: 5308-5319 [Abstract] [Full Text]  
  • Kikuchi, Y., Hosokawa, T., Fukatsu, T. (2007). Insect-Microbe Mutualism without Vertical Transmission: a Stinkbug Acquires a Beneficial Gut Symbiont from the Environment Every Generation. Appl. Environ. Microbiol. 73: 4308-4316 [Abstract] [Full Text]  
  • Sprent, J. I., James, E. K. (2007). Legume Evolution: Where Do Nodules and Mycorrhizas Fit In?. Plant Physiol. 144: 575-581 [Full Text]  
  • Malott, R. J., Sokol, P. A. (2007). Expression of the bviIR and cepIR Quorum-Sensing Systems of Burkholderia vietnamiensis. J. Bacteriol. 189: 3006-3016 [Abstract] [Full Text]  
  • Chain, P. S. G., Denef, V. J., Konstantinidis, K. T., Vergez, L. M., Agullo, L., Reyes, V. L., Hauser, L., Cordova, M., Gomez, L., Gonzalez, M., Land, M., Lao, V., Larimer, F., LiPuma, J. J., Mahenthiralingam, E., Malfatti, S. A., Marx, C. J., Parnell, J. J., Ramette, A., Richardson, P., Seeger, M., Smith, D., Spilker, T., Sul, W. J., Tsoi, T. V., Ulrich, L. E., Zhulin, I. B., Tiedje, J. M. (2006). Inaugural Article: Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility. Proc. Natl. Acad. Sci. USA 103: 15280-15287 [Abstract] [Full Text]  
  • Perin, L., Martinez-Aguilar, L., Paredes-Valdez, G., Baldani, J. I., Estrada-de los Santos, P., Reis, V. M., Caballero-Mellado, J. (2006). Burkholderia silvatlantica sp. nov., a diazotrophic bacterium associated with sugar cane and maize.. Int. J. Syst. Evol. Microbiol. 56: 1931-1937 [Abstract] [Full Text]  
  • Perin, L., Martinez-Aguilar, L., Castro-Gonzalez, R., Estrada-de los Santos, P., Cabellos-Avelar, T., Guedes, H. V., Reis, V. M., Caballero-Mellado, J. (2006). Diazotrophic burkholderia species associated with field-grown maize and sugarcane.. Appl. Environ. Microbiol. 72: 3103-3110 [Abstract] [Full Text]  
  • Chen, W.-M., de Faria, S. M., Straliotto, R., Pitard, R. M., Simoes-Araujo, J. L., Chou, J.-H., Chou, Y.-J., Barrios, E., Prescott, A. R., Elliott, G. N., Sprent, J. I., Young, J. P. W., James, E. K. (2005). Proof that Burkholderia Strains Form Effective Symbioses with Legumes: a Study of Novel Mimosa-Nodulating Strains from South America. Appl. Environ. Microbiol. 71: 7461-7471 [Abstract] [Full Text]  
  • Kikuchi, Y., Meng, X.-Y., Fukatsu, T. (2005). Gut Symbiotic Bacteria of the Genus Burkholderia in the Broad-Headed Bugs Riptortus clavatus and Leptocorisa chinensis (Heteroptera: Alydidae). Appl. Environ. Microbiol. 71: 4035-4043 [Abstract] [Full Text]  
  • Sessitsch, A., Coenye, T., Sturz, A. V., Vandamme, P., Barka, E. A., Salles, J. F., Van Elsas, J. D., Faure, D., Reiter, B., Glick, B. R., Wang-Pruski, G., Nowak, J. (2005). Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties. Int. J. Syst. Evol. Microbiol. 55: 1187-1192 [Abstract] [Full Text]  
  • Ramette, A., LiPuma, J. J., Tiedje, J. M. (2005). Species Abundance and Diversity of Burkholderia cepacia Complex in the Environment. Appl. Environ. Microbiol. 71: 1193-1201 [Abstract] [Full Text]  
  • Valdes, M., Perez, N.-O., Estrada-de los Santos, P., Caballero-Mellado, J., Pena-Cabriales, J. J., Normand, P., Hirsch, A. M. (2005). Non-Frankia Actinomycetes Isolated from Surface-Sterilized Roots of Casuarina equisetifolia Fix Nitrogen. Appl. Environ. Microbiol. 71: 460-466 [Abstract] [Full Text]  
  • Reis, V. M., Santos, P. E.-d. l., Tenorio-Salgado, S., Vogel, J., Stoffels, M., Guyon, S., Mavingui, P., Baldani, V. L. D., Schmid, M., Baldani, J. I., Balandreau, J., Hartmann, A., Caballero-Mellado, J. (2004). Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium. Int. J. Syst. Evol. Microbiol. 54: 2155-2162 [Abstract] [Full Text]  
  • Opelt, K., Berg, G. (2004). Diversity and Antagonistic Potential of Bacteria Associated with Bryophytes from Nutrient-Poor Habitats of the Baltic Sea Coast. Appl. Environ. Microbiol. 70: 6569-6579 [Abstract] [Full Text]  
  • Goris, J., De Vos, P., Caballero-Mellado, J., Park, J., Falsen, E., Quensen, J. F. III, Tiedje, J. M., Vandamme, P. (2004). Classification of the biphenyl- and polychlorinated biphenyl-degrading strain LB400T and relatives as Burkholderia xenovorans sp. nov.. Int. J. Syst. Evol. Microbiol. 54: 1677-1681 [Abstract] [Full Text]  
  • Salles, J. F., van Veen, J. A., van Elsas, J. D. (2004). Multivariate Analyses of Burkholderia Species in Soil: Effect of Crop and Land Use History. Appl. Environ. Microbiol. 70: 4012-4020 [Abstract] [Full Text]  
  • Caballero-Mellado, J., Martinez-Aguilar, L., Paredes-Valdez, G., Santos, P. E.-d. l. (2004). Burkholderia unamae sp. nov., an N2-fixing rhizospheric and endophytic species. Int. J. Syst. Evol. Microbiol. 54: 1165-1172 [Abstract] [Full Text]  
  • Martinez-Morales, F., Schobert, M., Lopez-Lara, I. M., Geiger, O. (2003). Pathways for phosphatidylcholine biosynthesis in bacteria. Microbiology 149: 3461-3471 [Abstract] [Full Text]  
  • Levy, A., Chang, B. J., Abbott, L. K., Kuo, J., Harnett, G., Inglis, T. J. J. (2003). Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp.. Appl. Environ. Microbiol. 69: 6250-6256 [Abstract] [Full Text]  
  • Hill, J. E., Seipp, R. P., Betts, M., Hawkins, L., Van Kessel, A. G., Crosby, W. L., Hemmingsen, S. M. (2002). Extensive Profiling of a Complex Microbial Community by High-Throughput Sequencing. Appl. Environ. Microbiol. 68: 3055-3066 [Abstract] [Full Text]  
  • Salles, J. F., De Souza, F. A., van Elsas, J. D. (2002). Molecular Method To Assess the Diversity of Burkholderia Species in Environmental Samples. Appl. Environ. Microbiol. 68: 1595-1603 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»