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Applied and Environmental Microbiology, June 2001, p. 2819-2822, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2819-2822.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Incorporation of [15N]Ammonia by the Cellulolytic Ruminal Bacteria Fibrobacter succinogenes BL2, Ruminococcus albus SY3, and Ruminococcus flavefaciens 17

Cengiz Atasoglu, C. James Newbold, and R. John Wallace*

Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom

Received 17 November 2000/Accepted 9 March 2001

The origin of cell nitrogen and amino acid nitrogen during growth of ruminal cellulolytic bacteria in different growth media was investigated by using 15NH3. At high concentrations of peptides (Trypticase, 10 g/liter) and amino acids (15.5 g/liter), significant amounts of cell nitrogen of Fibrobacter succinogenes BL2 (51%), Ruminococcus flavefaciens 17 (43%), and Ruminococcus albus SY3 (46%) were derived from non-NH3-N. With peptides at 1 g/liter, a mean of 80% of cell nitrogen was from NH3. More cell nitrogen was formed from NH3 during growth on cellobiose compared with growth on cellulose in all media. Phenylalanine was essential for F. succinogenes, and its 15N enrichment declined more than that of other amino acids in all species when amino acids were added to the medium.

* Corresponding author. Mailing address: Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom. Phone: 44 1224 716656. Fax: 44 1224 716687. E-mail: RJW{at}RRI.SARI.AC.UK.

Applied and Environmental Microbiology, June 2001, p. 2819-2822, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2819-2822.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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