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Applied and Environmental Microbiology, June 2001, p. 2823-2828, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2823-2828.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Fiber-Optic Fluorometer for Microscale Mapping of Photosynthetic Pigments in Microbial Communities

Roland Thar,1 Michael Kühl,1,* and Gerhard Holst2

Marine Biological Laboratory, University of Copenhagen, DK-3000 Helsingør, Denmark,1 and Max Planck Institute for Marine Microbiology, D-28359 Bremen, Germany2

Received 28 November 2000/Accepted 12 March 2001

Microscale fluorescence measurements were performed in photosynthetic biofilms at a spatial resolution of 100 to 200 µm with a new fiber-optic fluorometer which allowed four different excitation and emission wavelengths and was configured for measuring phycobiliproteins, chlorophylls, and bacteriochlorophylls. We present details of the measuring system and describe examples of applications in different microbial communities.

* Corresponding author. Mailing address: Marine Biological Laboratory, University of Copenhagen, Strandpromenaden 5, DK-3000 Helsingør, Denmark. Phone: 45 49 21 33 44. Fax: 45 49 26 11 65. E-mail: mkuhl{at}zi.ku.dk.

Applied and Environmental Microbiology, June 2001, p. 2823-2828, Vol. 67, No. 6
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.6.2823-2828.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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