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Applied and Environmental Microbiology, July 2001, p. 3053-3057, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3053-3057.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cattle Water Troughs as Reservoirs of Escherichia coli O157

Jeffrey T. LeJeune,1 Thomas E. Besser,1,* and Dale D. Hancock2

Department of Veterinary Microbiology and Pathology1 and Department of Veterinary Clinical Sciences,2 Washington State University, Pullman, Washington 99164

Received 20 September 2000/Accepted 25 April 2001

Environmental survival of Escherichia coli O157 may play an important role in the persistence and dissemination of this organism on farms. The survival of culturable and infectious E. coli O157 was studied using microcosms simulating cattle water troughs. Culturable E. coli O157 survived for at least 245 days in the microcosm sediments. Furthermore, E. coli O157 strains surviving more than 6 months in contaminated microcosms were infectious to a group of 10-week-old calves. Fecal excretion of E. coli O157 by these calves persisted for 87 days after challenge. Water trough sediments contaminated with feces from cattle excreting E. coli O157 may serve as a long-term reservoir of this organism on farms and a source of infection for cattle.

* Corresponding author. Mailing address: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164. Phone: (509) 335-6075. Fax: (509) 335-8529. E-mail: tbesser{at}vetmed.wsu.edu.

Applied and Environmental Microbiology, July 2001, p. 3053-3057, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3053-3057.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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