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Applied and Environmental Microbiology, July 2001, p. 3319-3321, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3319-3321.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Exo-Arabinanase of Penicillium chrysogenum Able To Release Arabinobiose from alpha -1,5-L-Arabinan

Tatsuji Sakamoto1,* and Jean-François Thibault2

Division of Applied Biochemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Osaka 599-8531, Japan,1 and Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, Institut National de la Recherche Agronomique, BP 71627-44316 Nantes Cedex 3, France2

Received 2 February 2001/Accepted 24 April 2001

An exo-arabinanase, designated Abnx, was purified from a culture filtrate of Penicillium chrysogenum 31B by ammonium sulfate precipitation, anion-exchange chromatography, and hydrophobic chromatography. Abnx had an apparent molecular mass of 47 kDa. The enzyme released only arabinobiose from the nonreducing terminus of alpha -1,5-L-arabinan and showed no activity towards p-nitrophenyl-alpha -L-arabinofuranoside and alpha -1,5-L-arabinofuranobiose. Abnx is the first enzyme with this mode of action.

* Corresponding author. Present address: Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, Institut National de la Recherche Agronomique, Rue de la Géraudière, BP 71627-44316 Nantes Cedex 3, France. Phone: 33 2 40 67 50 67. Fax: 33 2 40 67 50 66. E-mail: sakamoto{at}nantes.inra.fr or sakamoto{at}biochem.osakafu-u.ac.jp.

Applied and Environmental Microbiology, July 2001, p. 3319-3321, Vol. 67, No. 7
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.7.3319-3321.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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