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Applied and Environmental Microbiology, August 2001, p. 3455-3462, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3455-3462.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Isolation and Characterization of a Gene Specific to Lager Brewing Yeast That Encodes a Branched-Chain Amino Acid Permease

Yukiko Kodama,^* Fumihiko Omura, and Toshihiko Ashikari

Institute for Fundamental Research, Suntory Research Center, Mishima-gun, Osaka 618-8503, Japan

Received 1 March 2001/Accepted 30 May 2001

We found two types of branched-chain amino acid permease gene (BAP2) in the lager brewing yeast Saccharomyces pastorianus BH-225 and cloned one type of BAP2 gene (Lg-BAP2), which is identical to that of Saccharomyces bayanus (by-BAP2-1). The other BAP2 gene of the lager brewing yeast (cer-BAP2) is very similar to the Saccharomyces cerevisiae BAP2 gene. This result substantiates the notion that lager brewing yeast is a hybrid of S. cerevisiae and S. bayanus. The amino acid sequence homology between S. cerevisiae Bap2p and Lg-Bap2p was 88%. The transcription of Lg-BAP2 was not induced by the addition of leucine to the growth medium, while that of cer-BAP2 was induced. The transcription of Lg-BAP2 was repressed by the presence of ethanol and weak organic acid, while that of cer-BAP2 was not affected by these compounds. Furthermore, Northern analysis during beer fermentation revealed that the transcription of Lg-BAP2 was repressed at the beginning of the fermentation, while cer-BAP2 was highly expressed throughout the fermentation. These results suggest that the transcription of Lg-BAP2 is regulated differently from that of cer-BAP2 in lager brewing yeasts.

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^* Corresponding author. Mailing address: Institute for Fundamental Research, Suntory Research Center, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-6503, Japan. Phone: 81-75-962-8802. Fax: 81-75-962-8262. E-mail: Yukiko_Kodama{at}suntory.co.jp.

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Applied and Environmental Microbiology, August 2001, p. 3455-3462, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3455-3462.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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