Previous Article | Next Article 
Applied and Environmental Microbiology, August 2001, p. 3469-3475, Vol. 67, No. 8
VTT Biotechnology, FIN-02044 VTT Espoo,
Finland
Received 22 December 2000/Accepted 9 May 2001
Adhesion of 19 Bifidobacterium strains to native
maize, potato, oat, and barley starch granules was examined to
investigate links between adhesion and substrate utilization and to
determine if adhesion to starch could be exploited in probiotic food
technologies. Starch adhesion was not characteristic of all the
bifidobacteria tested. Adherent bacteria bound similarly to the
different types of starch, and the binding capacity of the starch
(number of bacteria per gram) correlated to the surface area of the
granules. Highly adherent strains were able to hydrolyze the granular
starches, but not all amylolytic strains were adherent, indicating that starch adhesion is not a prerequisite for efficient substrate utilization for all bifidobacteria. Adhesion was mediated by a cell
surface protein(s). For the model organisms tested
(Bifidobacterium adolescentis VTT E-001561 and
Bifidobacterium pseudolongum ATCC 25526), adhesion
appeared to be specific for
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.8.3469-3475.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Adhesion of Bifidobacteria to Granular Starch
and Its Implications in Probiotic Technologies
-1,4-linked glucose sugars, since
adhesion was inhibited by maltose, maltodextrin, amylose, and soluble
starch but not by trehalose, cellobiose, or lactose. In an in vitro
gastric model, adhesion was inhibited both by the action of protease
and at pH values of 
3. Adhesion was not affected by bile, but the
binding capacity of the starch was reduced by exposure to pancreatin.
It may be possible to exploit adhesion of probiotic bifidobacteria to
starch granules in microencapsulation technology and for synbiotic food applications.
*
Corresponding author. Present address: 16/5
Coleridge St., Elwood, Victoria 3184, Australia. Phone:
61-3-95315191. E-mail: crittenden{at}visto.com.
Applied and Environmental Microbiology, August 2001, p. 3469-3475, Vol. 67, No. 8
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.8.3469-3475.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Worthley, D. L, Le Leu, R. K, Whitehall, V. L, Conlon, M., Christophersen, C., Belobrajdic, D., Mallitt, K.-A., Hu, Y., Irahara, N., Ogino, S., Leggett, B. A, Young, G. P
(2009). A human, double-blind, placebo-controlled, crossover trial of prebiotic, probiotic, and synbiotic supplementation: effects on luminal, inflammatory, epigenetic, and epithelial biomarkers of colorectal cancer. Am. J. Clin. Nutr.
90: 578-586
[Abstract] [Full Text] -
Ryan, S. M., Fitzgerald, G. F., van Sinderen, D.
(2006). Screening for and Identification of Starch-, Amylopectin-, and Pullulan-Degrading Activities in Bifidobacterial Strains. Appl. Environ. Microbiol.
72: 5289-5296
[Abstract] [Full Text] -
Valerio, F., De Bellis, P., Lonigro, S. L., Morelli, L., Visconti, A., Lavermicocca, P.
(2006). In Vitro and In Vivo Survival and Transit Tolerance of Potentially Probiotic Strains Carried by Artichokes in the Gastrointestinal Tract. Appl. Environ. Microbiol.
72: 3042-3045
[Abstract] [Full Text] -
Lavermicocca, P., Valerio, F., Lonigro, S. L., De Angelis, M., Morelli, L., Callegari, M. L., Rizzello, C. G., Visconti, A.
(2005). Study of Adhesion and Survival of Lactobacilli and Bifidobacteria on Table Olives with the Aim of Formulating a New Probiotic Food. Appl. Environ. Microbiol.
71: 4233-4240
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»