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Applied and Environmental Microbiology, August 2001, p. 3610-3617, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3610-3617.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Degradation of Chlorinated Dibenzofurans and Dibenzo-p-Dioxins by Two Types of Bacteria Having Angular Dioxygenases with Different Features

Hiroshi Habe,1 Jin-Sung Chung,1 Jong-Hoon Lee,2 Kano Kasuga,3 Takako Yoshida,1 Hideaki Nojiri,1 and Toshio Omori1,*

Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657,1 and Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Shimoshinjo-nakano, Akita 010-0195,3 Japan, and Department of Food Science and Biotechnology, Kyonggi University, Suwon, Kyonggi-Do 442-760, Korea2

Received 28 February 2001/Accepted 18 May 2001

Two kinds of bacteria having different-structured angular dioxygenases---a dibenzofuran (DF)-utilizing bacterium, Terrabacter sp. strain DBF63, and a carbazole (CAR)-utilizing bacterium, Pseudomonas sp. strain CA10---were investigated for their ability to degrade some chlorinated dibenzofurans (CDFs) and chlorinated dibenzo-p-dioxins (CDDs) (or, together, CDF/Ds) using either wild-type strains or recombinant Escherichia coli strains. First, it was shown that CAR 1,9a-dioxygenase (CARDO) catalyzed angular dioxygenation of all mono- to triCDF/Ds investigated in this study, but DF 4,4a-dioxygenase (DFDO) did not degrade 2,7-diCDD. Secondly, degradation of CDF/Ds by the sets of three enzymes (angular dioxygenase, extradiol dioxygenase, and meta-cleavage compound hydrolase) was examined, showing that these enzymes in both strains were able to convert 2-CDF to 5-chlorosalicylic acid but not other tested substrates to the corresponding chlorosalicylic acid (CSA) or chlorocatechol (CC). Finally, we tested the potential of both wild-type strains for cooxidation of CDF/Ds and demonstrated that both strains degraded 2-CDF, 2-CDD, and 2,3-diCDD to the corresponding CSA and CC. We investigated the sites for the attack of angular dioxygenases in each CDF/D congener, suggesting the possibility that the angular dioxygenation of 2-CDF, 2-CDD, 2,3-diCDD, and 1,2,3-triCDD (10 ppm each) by both DFDO and CARDO occurred mainly on the nonsubstituted aromatic nuclei.

* Corresponding author. Mailing address: Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Phone: 81(3)5841-3067. Fax: 81(3)5841-8030. E-mail: aseigyo{at}mail.ecc.u-tokyo.ac.jp.

Applied and Environmental Microbiology, August 2001, p. 3610-3617, Vol. 67, No. 8
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.8.3610-3617.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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