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Applied and Environmental Microbiology, September 2001, p. 3970-3975, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.3970-3975.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Direct Incorporation of Glucosamine and N-Acetylglucosamine into Exopolymers by Gluconacetobacter xylinus (=Acetobacter xylinum) ATCC 10245: Production of Chitosan-Cellulose and Chitin-Cellulose Exopolymers

Jin W. Lee,1 Fang Deng,2 Walter G. Yeomans,3 Alfred L. Allen,3 Richard A. Gross,4,* and David L. Kaplan5,*

Dong-A University, Hadan 2-dong, Sha-gu, Pusan 604-714, Korea1; Department of Chemistry, University of Massachusetts, Lowell, Massachusetts 018542; Biotechnology Division, U.S. Army Natick RD & E Center, Natick, Massachusetts 017603; Polytechnic University, Brooklyn, New York4; and Department of Chemical & Biological Engineering, Biotechnology Center, Tufts University, Medford, Massachusetts 021555

Received 19 April 2001/Accepted 17 June 2001

Gluconacetobacter xylinus (=Acetobacter xylinum) ATCC 10245 incorporated 2-amino-2-deoxy-D-glucose (glucosamine) and 2-acetamido-2-deoxy-D-glucose (N-acetylglucosamine), but not 3-O-methyl-D-glucose or 2-deoxy-D-glucose into exopolymers. Incorporation was confirmed by gas chromatography with and without mass spectrometry, Fourier transform infrared, and 1H nuclear magnetic resonance. The average molar percentage of glucosamine and N-acetylglucosamine in the exopolymers was about 18%.

* Corresponding author. Mailing address for Richard A. Gross: Department of Chemistry, Polytechnic University, Six Metrotech Center, Brooklyn, NY 11201. E-mail: gros{at}poly.edu. Mailing address for David L. Kaplan: Department of Chemical & Biological Engineering, Biotechnology Center, Tufts University, 4 Colby Street, Medford, MA 02155. Phone: (617) 627-3251. Fax: (617) 627-3991. E-mail: david.kaplan{at}tufts.edu.

Applied and Environmental Microbiology, September 2001, p. 3970-3975, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.3970-3975.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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