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Applied and Environmental Microbiology, September 2001, p. 4137-4143, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4137-4143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Survival of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in the Terminal Ileum of Fistulated Göttingen Minipigs

Sonja Lick,1,* Karsten Drescher,2 and Knut J. Heller1

Institute for Microbiology1 and Institute for Physiology and Biochemistry of Nutrition,2 Federal Dairy Research Center, D-24103 Kiel, Germany

Received 5 February 2001/Accepted 29 June 2001

The ability of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus administered in yogurt to survive the passage through the upper gastrointestinal tract was investigated with Göttingen minipigs that were fitted with ileum T-cannulas. After ingestion of yogurt containing viable microorganisms, ileostomy samples were collected nearly every hour beginning 3 h after food uptake. Living L. delbrueckii subsp. bulgaricus and S. thermophilus were detected in the magnitude of 106 to 107 per gram of intestinal contents (wet weight) in all animals under investigation. A calculation of the minimum amount of surviving bacteria that had been administered is presented. Total DNA extracted from ileostomy samples was subjected to PCR, which was species specific for L. delbrueckii and S. thermophilus and subspecies specific for L. delbrueckii subsp. bulgaricus. All three bacterial groups could be detected by PCR after yogurt uptake but not after uptake of a semisynthetic diet. One pig apparently had developed an endogenous L. delbrueckii flora. When heat-treated yogurt was administered, L. delbrueckii was detected in all animals. S. thermophilus or L. delbrueckii subsp. bulgaricus was not detected, indicating that heat-inactivated cells and their DNAs had already been digested and their own L. delbrueckii flora had been stimulated for growth.

* Corresponding author. Mailing address: Institute for Microbiology, Federal Dairy Research Center, Kiel, Hermann-Weigmann-Str. 1, D-24103 Kiel, Germany. Phone: 49-431-609-2351. Fax: 49-431-609-2306. E-mail: lick{at}bafm.de.

Applied and Environmental Microbiology, September 2001, p. 4137-4143, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4137-4143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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