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Applied and Environmental Microbiology, September 2001, p. 4365-4371, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4365-4371.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Diversity and Distribution in Hypersaline Microbial Mats of Bacteria Related to Chloroflexus spp.

Ulrich Nübel,1,* Mary M. Bateson,1 Michael T. Madigan,2 Michael Kühl,3 and David M. Ward1

Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, Montana1; Department of Microbiology, Southern Illinois University, Carbondale, Illinois2; and Marine Biological Laboratory, University of Copenhagen, Helsingør, Denmark3

Received 13 February 2001/Accepted 2 July 2001

Filamentous bacteria containing bacteriochlorophylls c and a were enriched from hypersaline microbial mats. Based on phylogenetic analyses of 16S rRNA gene sequences, these organisms form a previously undescribed lineage distantly related to Chloroflexus spp. We developed and tested a set of PCR primers for the specific amplification of 16S rRNA genes from filamentous phototrophic bacteria within the kingdom of "green nonsulfur bacteria." PCR products recovered from microbial mats in a saltern in Guerrero Negro, Mexico, were subjected to cloning or denaturing gradient gel electrophoresis and then sequenced. We found evidence of a high diversity of bacteria related to Chloroflexus which exhibit different distributions along a gradient of salinity from 5.5 to 16%.

* Corresponding author. Present address: Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1B, 38124 Braunschweig, Germany. Phone: 49-531-2616-105. Fax: 49-531-2616-418. E-mail: unuebel{at}dsmz.de.

Applied and Environmental Microbiology, September 2001, p. 4365-4371, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4365-4371.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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