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Applied and Environmental Microbiology, September 2001, p. 4372-4373, Vol. 67, No. 9
Department of Biological Sciences, Imperial
College of Science, Technology, and Medicine, Silwood Park, Ascot,
Berkshire SL5 7PY,1 and School of
Biological Sciences, University of Sussex, Falmer, Brighton, East
Sussex BN1 9QG,2 United Kingdom
Received 7 May 2001/Accepted 19 June 2001
Resistant and susceptible populations of the diamondback moth
(Plutella xylostella) were tested with crystalline,
solubilized, and partially and fully activated forms of the
Bacillus thuringiensis Cry1Ac
0099-2240/01/$04.00+0 DOI: 10.1128/AEM.67.9.4372-4373.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Susceptibility of a Field-Derived, Bacillus
thuringiensis-Resistant Strain of Diamondback Moth to In
Vitro-Activated Cry1Ac Toxin
-endotoxin. Fully
activated toxin greatly reduced the resistance ratio (ratio of the 50%
lethal concentration for the resistant population to that for the
susceptible population) of the resistant population, suggesting
that a defect in toxin activation is a major resistance mechanism.
*
Corresponding author. Mailing address: School
of Biological Sciences, University of Sussex, Falmer, Brighton, East
Sussex BN1 9QG, United Kingdom. Phone: 44 1273 678917. Fax: 44 1273 678433. E-mail: n.crickmore{at}sussex.ac.uk.
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