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Applied and Environmental Microbiology, September 2001, p. 4372-4373, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4372-4373.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Susceptibility of a Field-Derived, Bacillus thuringiensis-Resistant Strain of Diamondback Moth to In Vitro-Activated Cry1Ac Toxin

Ali H. Sayyed,1 Roxani Gatsi,2 Thaleia Kouskoura,2 Denis J. Wright,1 and Neil Crickmore2,*

Department of Biological Sciences, Imperial College of Science, Technology, and Medicine, Silwood Park, Ascot, Berkshire SL5 7PY,1 and School of Biological Sciences, University of Sussex, Falmer, Brighton, East Sussex BN1 9QG,2 United Kingdom

Received 7 May 2001/Accepted 19 June 2001

Resistant and susceptible populations of the diamondback moth (Plutella xylostella) were tested with crystalline, solubilized, and partially and fully activated forms of the Bacillus thuringiensis Cry1Ac delta -endotoxin. Fully activated toxin greatly reduced the resistance ratio (ratio of the 50% lethal concentration for the resistant population to that for the susceptible population) of the resistant population, suggesting that a defect in toxin activation is a major resistance mechanism.

* Corresponding author. Mailing address: School of Biological Sciences, University of Sussex, Falmer, Brighton, East Sussex BN1 9QG, United Kingdom. Phone: 44 1273 678917. Fax: 44 1273 678433. E-mail: n.crickmore{at}sussex.ac.uk.

Applied and Environmental Microbiology, September 2001, p. 4372-4373, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4372-4373.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sayyed, A. H., Gatsi, R., Ibiza-Palacios, M. S., Escriche, B., Wright, D. J., Crickmore, N. (2005). Common, but Complex, Mode of Resistance of Plutella xylostella to Bacillus thuringiensis Toxins Cry1Ab and Cry1Ac. Appl. Environ. Microbiol. 71: 6863-6869 [Abstract] [Full Text]  
  • Raymond, B., Sayyed, A. H, Wright, D. J (2005). Genes and environment interact to determine the fitness costs of resistance to Bacillus thuringiensis. Proc R Soc B 272: 1519-1524 [Abstract] [Full Text]  
  • Sayyed, A. H., Raymond, B., Ibiza-Palacios, M. S., Escriche, B., Wright, D. J. (2004). Genetic and Biochemical Characterization of Field-Evolved Resistance to Bacillus thuringiensis Toxin Cry1Ac in the Diamondback Moth, Plutella xylostella. Appl. Environ. Microbiol. 70: 7010-7017 [Abstract] [Full Text]  
  • Jurat-Fuentes, J. L., Gould, F. L., Adang, M. J. (2003). Dual Resistance to Bacillus thuringiensis Cry1Ac and Cry2Aa Toxins in Heliothis virescens Suggests Multiple Mechanisms of Resistance. Appl. Environ. Microbiol. 69: 5898-5906 [Abstract] [Full Text]  
  • Candas, M., Loseva, O., Oppert, B., Kosaraju, P., Bulla, L. A. Jr. (2003). Insect Resistance to Bacillus thuringiensis: Alterations in the Indianmeal Moth Larval Gut Proteome. Mol. Cell. Proteomics 2: 19-28 [Abstract] [Full Text]  


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