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Applied and Environmental Microbiology, September 2001, p. 4390-4392, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4390-4392.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

In Vivo 23Na Nuclear Magnetic Resonance Study of Maintenance of a Sodium Gradient in the Ruminal Bacterium Fibrobacter succinogenes S85

Véronique Schwaab,1,2 Christelle Matheron,1 Anne-Marie Delort,1,* Geneviève Gaudet,2 and Evelyne Forano2

Laboratoire de Synthèse, Electrosynthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504, Université Blaise Pascal---CNRS, 63177 Aubière cedex,1 and Unité de Microbiologie, INRA, Centre de Recherches de Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle,2 France

Received 26 January 2001/Accepted 7 June 2001

Sodium gradients (Delta pNa) were measured in resting cells of Fibrobacter succinogenes by in vivo 23Na nuclear magnetic resonance using Tm(DOTP)5- [thulium(III) 1,4,7,10-tetraazacyclododecane-N',N'',N'''-tetramethylenephosphonate] as the shift reagent. This bacterium was able to maintain a Delta pNa of -55 to -40 mV for extracellular sodium concentrations ranging from 30 to 200 mM. Depletion of Na+ ions during the washing steps led to irreversible damage (modification of glucose metabolism and inability to maintain a sodium gradient).

* Corresponding author. Mailing address: Laboratoire de Synthèse, Electrosynthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504, Université Blaise Pascal-CNRS, 63177 Aubière cedex, France. Phone: 33 04 73 40 77 14. Fax: 33 04 73 40 77 17. E-mail: amdelort{at}chimtp.univ-bpclermont.fr.

Applied and Environmental Microbiology, September 2001, p. 4390-4392, Vol. 67, No. 9
0099-2240/01/$04.00+0   DOI: 10.1128/AEM.67.9.4390-4392.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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