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Applied and Environmental Microbiology, January 2002, p. 408-411, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.408-411.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cloning and Characterization of the Crystal Protein-Encoding Gene of Bacillus thuringiensis subsp. yunnanensis

Periasamy Balasubramanian, R. Jayakumar, Prashant Shambharkar, N. Unnamalai, S. Karutha Pandian, N. Selvamuthu Kumaraswami, R. Ilangovan, and Vaithilingam Sekar*

Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India

Received 20 August 2001/ Accepted 25 October 2001

Molecular cloning and characterization of a novel cry gene, cry32Aa, of Bacillus thuringiensis subsp. yunnanensis was carried out. The Cry32Aa protein was predicted to have a molecular mass of 139.2 kDa and was found to have an unusual 42-amino-acid-long tail at the C terminus. The cry32Aa gene was localized on the 103-MDa plasmid of the organism. Bioassays showed no toxicity against several moths and mosquitoes. However, it exhibited weak toxicity against larvae of the diamondback moth, Plutella xylostella.

* Corresponding author. Mailing address: Dept. of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India. Phone: 91-452-858684. Fax: 91-452-859105. E-mail: vsekar{at}mrna.tn.nic.in.

Applied and Environmental Microbiology, January 2002, p. 408-411, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.408-411.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guo, S., Liu, M., Peng, D., Ji, S., Wang, P., Yu, Z., Sun, M. (2008). New Strategy for Isolating Novel Nematicidal Crystal Protein Genes from Bacillus thuringiensis Strain YBT-1518. Appl. Environ. Microbiol. 74: 6997-7001 [Abstract] [Full Text]  


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