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Applied and Environmental Microbiology, January 2002, p. 46-52, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.46-52.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with Various Plasmids

Pascale Serror,1* Takashi Sasaki,2 S. Dusko Ehrlich,1 and Emmanuelle Maguin1

Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France,1 Food Functionality Research Institute, Meiji Milk Products Co., Ltd., 540 Naruda, Odawara 250-0862, Japan2

Received 11 December 2000/ Accepted 1 October 2001

We describe, for the first time, a detailed electroporation procedure for Lactobacillus delbrueckii. Three L. delbrueckii strains were successfully transformed. Under optimal conditions, the transformation efficiency was 104 transformants per µg of DNA. Using this procedure, we identified several plasmids able to replicate in L. delbrueckii and integrated an integrative vector based on phage integrative elements into the L. delbrueckii subsp. bulgaricus chromosome. These vectors provide a good basis for developing molecular tools for L. delbrueckii and open the field of genetic studies in L. delbrueckii.

* Corresponding author. Mailing address: Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France. Phone: 33-1-34 65 25 31. Fax: 33-1-34 65 25 21. E-mail: serror{at}diamant.jouy.inra.fr.

Applied and Environmental Microbiology, January 2002, p. 46-52, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.46-52.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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