This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guedon, E.
Right arrow Articles by Petitdemange, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guedon, E.
Right arrow Articles by Petitdemange, H.
Agricola
Right arrow Articles by Guedon, E.
Right arrow Articles by Petitdemange, H.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2002, p. 53-58, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.53-58.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Improvement of Cellulolytic Properties of Clostridium cellulolyticum by Metabolic Engineering

Emmanuel Guedon,{dagger} Mickaël Desvaux, and Henri Petitdemange*

Laboratoire de Biochimie des Bactéries Gram Positif, Faculté des Sciences, Université Henri Poincaré, 54506 Vandoeuvre-lès-Nancy Cedex, France

Received 26 July 2001/ Accepted 2 October 2001

Cellulolytic clostridia have evolved to catabolize lignocellulosic materials at a seasonal biorhythm, so their biotechnological exploitation requires genetic improvements. As high carbon flux leads to pyruvate accumulation, which is responsible for the cessation of growth of Clostridium cellulolyticum, this accumulation is decreased by heterologous expression of pyruvate decarboxylase and alcohol dehydrogenase from Zymomonas mobilis. In comparison with that of the wild strain, growth of the recombinant strain at the same specific rate but for 145 h instead of 80 h led to a 150% increase in cellulose consumption and a 180% increase in cell dry weight. The fermentation pattern was shifted significantly: lactate production decreased by 48%, whereas the concentrations of acetate and ethanol increased by 93 and 53%, respectively. This study demonstrates that the fermentation of cellulose, the most abundant and renewable polymer on earth, can be greatly improved by using genetically engineered C. cellulolyticum.

* Corresponding author. Mailing address: Laboratoire de Biochimie des Bactéries Gram Positif, Domaine Scientifique Victor Grignard, Université Henri Poincaré, Faculté des Sciences, BP 239, 54506 Vandoeuvre-lès-Nancy Cedex, France. Phone: 33 3 83 91 20 53. Fax: 33 3 83 91 25 50. E-mail: hpetitde{at}lcb.uhp-nancy.fr.

{dagger} Present address: Department of Microbiology, Cornell University, Ithaca, NY 14853-8101.

Applied and Environmental Microbiology, January 2002, p. 53-58, Vol. 68, No. 1
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.1.53-58.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mingardon, F., Perret, S., Belaich, A., Tardif, C., Belaich, J.-P., Fierobe, H.-P. (2005). Heterologous Production, Assembly, and Secretion of a Minicellulosome by Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 71: 1215-1222 [Abstract] [Full Text]  
  • Perret, S., Belaich, A., Fierobe, H.-P., Belaich, J.-P., Tardif, C. (2004). Towards Designer Cellulosomes in Clostridia: Mannanase Enrichment of the Cellulosomes Produced by Clostridium cellulolyticum. J. Bacteriol. 186: 6544-6552 [Abstract] [Full Text]  
  • Tyurin, M. V., Desai, S. G., Lynd, L. R. (2004). Electrotransformation of Clostridium thermocellum. Appl. Environ. Microbiol. 70: 883-890 [Abstract] [Full Text]  
  • Fujita, Y., Ito, J., Ueda, M., Fukuda, H., Kondo, A. (2004). Synergistic Saccharification, and Direct Fermentation to Ethanol, of Amorphous Cellulose by Use of an Engineered Yeast Strain Codisplaying Three Types of Cellulolytic Enzyme. Appl. Environ. Microbiol. 70: 1207-1212 [Abstract] [Full Text]  
  • Maamar, H., de Philip, P., Belaich, J.-P., Tardif, C. (2003). ISCce1 and ISCce2, Two Novel Insertion Sequences in Clostridium cellulolyticum. J. Bacteriol. 185: 714-725 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»