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Applied and Environmental Microbiology, October 2002, p. 4876-4883, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.4876-4883.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Glucose and Nutrient Concentrations Affect the Expression of a 104-Kilodalton Listeria Adhesion Protein in Listeria monocytogenes

Ziad W. Jaradat and Arun K. Bhunia*

Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, Indiana 47907

Received 8 March 2002/ Accepted 24 July 2002

Growth media and environmental conditions influence the expression of adhesion and invasion proteins in Listeria monocytogenes. Here, the expression of the 104-kDa Listeria adhesion protein (LAP) was studied in nutrient-rich media (Trypticase soy broth [TSB] and brain heart infusion [BHI]), minimal medium (Luria-Bertani [LB]), or nutrient-deficient medium (peptone water [PW]) by immunoblotting, enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy. Also, the effect of incorporating different concentrations of glucose on LAP expression was studied. Immunoblotting showed that LAP expression was at least twofold higher in LB medium than in TSB or BHI, while PW supported very poor cell growth and LAP expression. ELISA and immunoblotting results showed that higher concentrations of glucose (>1.6 g/liter) lowered the culture pH and suppressed LAP expression by more than 75%; however, the addition of K2HPO4 reduced this effect. L. monocytogenes cells grown in LB media with lower concentrations of glucose showed higher adhesion to Caco-2 cells (3,716 and 4,186 cpm of attached bacteria for 0 and 0.2 g of glucose/liter, respectively), while L. monocytogenes cells grown in LB with higher glucose concentrations exhibited lower adhesion (2,126 and 2,221 cpm for 1.6 and 3.2 g of glucose/liter, respectively). A LAP-negative L. monocytogenes strain (A572) showed low adhesion profiles regardless of the amount of glucose added. Transmission electron microscopy revealed that LAP is localized mainly in the cytoplasm, with only a few molecules located on the cell surface. Growth in LB with high glucose (3.2 g/liter) showed the presence of only a few molecules in the cells, corroborating the results observed with ELISA or immunoblotting. In summary, nutrient-rich media and high concentrations of glucose suppressed LAP expression, which possibly is due to the changes in the pH of the media during growth from the accumulation of sugar fermentation by-products.

* Corresponding author. Mailing address: Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907. Phone: (765) 494-5443. Fax: (765) 494-7953. E-mail: Bhuniaa{at}foodsci.purdue.edu.

Applied and Environmental Microbiology, October 2002, p. 4876-4883, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.4876-4883.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stritzker, J., Schoen, C., Goebel, W. (2005). Enhanced Synthesis of Internalin A in aro Mutants of Listeria monocytogenes Indicates Posttranscriptional Control of the inlAB mRNA. J. Bacteriol. 187: 2836-2845 [Abstract] [Full Text]  
  • Wampler, J. L., Kim, K.-P., Jaradat, Z., Bhunia, A. K. (2004). Heat Shock Protein 60 Acts as a Receptor for the Listeria Adhesion Protein in Caco-2 Cells. Infect. Immun. 72: 931-936 [Abstract] [Full Text]  
  • Jaradat, Z. W., Bhunia, A. K. (2003). Adhesion, Invasion, and Translocation Characteristics of Listeria monocytogenes Serotypes in Caco-2 Cell and Mouse Models. Appl. Environ. Microbiol. 69: 3640-3645 [Abstract] [Full Text]  


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