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Applied and Environmental Microbiology, October 2002, p. 4900-4905, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.4900-4905.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Bacillus thuringiensis in Fecal Samples from Greenhouse Workers after Exposure to B. thuringiensis-Based Pesticides

Gert B. Jensen,1* Preben Larsen,2 Bodil L. Jacobsen,3 Bodil Madsen,3 Lasse Smidt,1 and Lars Andrup1

Department of Chemical Working Environments, National Institute of Occupational Health, DK-2100 Copenhagen,1 Department of Occupational and Environmental Medicine, Odense University Hospital, DK-5000 Odense,2 The Danish Veterinary and Food Administration, DK-2860 Søborg, Denmark3

Received 1 April 2002/ Accepted 26 July 2002

In a study of occupational exposure to Bacillus thuringiensis, 20 exposed greenhouse workers were examined for Bacillus cereus-like bacteria in fecal samples and on biomonitoring filters. Bacteria with the following characteristics were isolated from eight individuals: intracellular crystalline inclusions characteristic of B. thuringiensis, genes for and production of B. cereus enterotoxins, and positivity for cry11 as determined by PCR. DNA fingerprints of the fecal isolates were identical to those of strains isolated from the commercial products used. Work processes (i.e., spraying) correlated with the presence of B. thuringiensis in the fecal samples (102 to 103 CFU/g of feces). However, no gastrointestinal symptoms correlated with the presence of B. thuringiensis in the fecal samples.

* Corresponding author. Mailing address: Department of Chemical Working Environments, National Institute of Occupational Health, Lersø Parkallé 105, DK-2100 Copenhagen, Denmark. Phone: (45) 3916 5244. Fax: (45) 3916 5201. E-mail: gbj{at}ami.dk.

Applied and Environmental Microbiology, October 2002, p. 4900-4905, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.4900-4905.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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