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Applied and Environmental Microbiology, October 2002, p. 5142-5150, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5142-5150.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Succession of Bacterial Community Structure along the Changjiang River Determined by Denaturing Gradient Gel Electrophoresis and Clone Library Analysis

Hiroyuki Sekiguchi,1,2 Masataka Watanabe,1 Tadaatsu Nakahara,2 Baohua Xu,3 and Hiroo Uchiyama2*

National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506,1 Institute of Applied Biochemistry, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan,2 Yangtze Valley Water Resources Protection Bureau, Wuhan, 430010, People's Republic of China3

Received 2 January 2002/ Accepted 12 July 2002

Bacterial community structure along the Changjiang River (which is more than 2,500 km long) was studied by using denaturing gradient gel electrophoresis (DGGE) and clone library analysis of PCR-amplified 16S ribosomal DNA (rDNA) with universal bacterial primer sets. DGGE profiles and principal-component analysis (PCA) demonstrated that the bacterial community gradually changed from upstream to downstream in both 1998 and 1999. Bacterial diversity, as determined by the Shannon index (H'), gradually decreased from upstream to downstream. The PCA plots revealed that the differences in the bacterial communities among riverine stations were not appreciable compared with the differences in two adjacent lakes, Lake Dongting and Lake Poyang. The relative stability of the bacterial communities at the riverine stations was probably due to the buffering action of the large amount of water flowing down the river. Clone library analysis of 16S rDNA revealed that the dominant bacterial groups changed from ß-proteobacteria and the Cytophaga-Flexibacter-Bacteroides group upstream to high-G+C-content gram-positive bacteria downstream and also that the bacterial community structure differed among the stations in the river and the lakes. The results obtained in this study should provide a reference for future changes caused by construction of the Three Gorges Dam.

* Corresponding author. Mailing address: Institute of Applied Biochemistry, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. Phone: 81-298-53-6626. Fax: 81-298-53-4605. E-mail: uchiyama{at}sakura.cc.tsukuba.ac.jp.

Applied and Environmental Microbiology, October 2002, p. 5142-5150, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5142-5150.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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